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Beckman Coulter JA-25.50 User Manual

Beckman Coulter JA-25.50
31 pages
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PN J-TB-056FD
September 2016
Instructions For Use
JA-25.50
Fixed-Angle Rotor
For Use in Beckman Coulter
J2 and Avanti J Series Centrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

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Beckman Coulter JA-25.50 Specifications

General IconGeneral
Maximum Speed25, 000 RPM
Maximum RCF62, 000 x g
MaterialAluminum
Rotor TypeFixed-angle
Tube Angle25°
Tube MaterialPolypropylene
CompatibilityBeckman Coulter centrifuges

Summary

Safety Information and Alerts

Alerts for Warning, Caution, Important, and Note

Explains different alert types (Warning, Caution, Important, Note) used in the manual.

Safety Information for the JA-25.50 Rotor

Provides crucial safety guidelines and precautions specific to the JA-25.50 rotor.

Rotor Specifications

Rotor Description and Features

Preparation and Usage Guide

Prerun Safety Checks

Lists essential checks to perform before using the rotor to ensure safety and proper function.

Rotor Preparation

Details on cleaning, lubricating, and preparing the rotor for loading samples.

Operation

Instructions on precooling the rotor and selecting the correct centrifuge settings.

Installing the Rotor

Step-by-step guide on how to properly install the rotor onto the centrifuge drive hub.

Temperature Settings

Explains how to set and control the temperature for runs in Avanti J and J2 series centrifuges.

Run Procedure

Outlines the final steps to initiate a centrifugation run after loading and installation.

Removal and Sample Recovery

Describes the safe procedures for removing the rotor and recovering samples after a run.

Tubes and Bottles

Lists compatible tubes and bottles, including fill volumes and speed limits.

Run Times Calculation

Provides the formula and example for calculating run times based on sedimentation coefficient.

Care, Maintenance, and Cleaning

Run Speeds and RCF

Discusses how run speed affects centrifugal force and defines relative centrifugal fields (RCF).

Routine Maintenance

Details periodic inspection of rotor cavities, drive pins, and lubrication of threads/O-rings.

Cleaning Procedures

Provides instructions for washing the rotor and its components using mild detergent and distilled water.

Decontamination Guidelines

Offers guidance on decontaminating the rotor if exposed to radioactive materials.

Sterilization and Disinfection

Outlines methods for autoclaving, using ethanol, or bleach for rotor sterilization and disinfection.

Proper Storage

Advises on storing the rotor in a dry environment with the lid removed to allow air circulation.

Returning a Rotor for Service

Explains the procedure and required information for returning a rotor for service or repair.

Replacement Parts and Supplies

Replacement Rotor Parts

Lists part numbers for rotor assemblies, lids, and O-rings.

Associated Supplies

Lists part numbers for cleaning kits, lubricants, and references compatible tubes.

J Series Rotor Warranty Details

Rotor Replacement Process

Details the process and conditions for obtaining a replacement for a defective rotor.

Warranty Conditions and Exclusions

Lists terms and exclusions that void the rotor's warranty.

Warranty Disclaimer

States limitations on warranties and liability for damages.

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