Beckman Coulter J2-HC User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

F
Fred MooreAug 17, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- D
  Dustin GordonAug 18, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
S
Sharon LloydAug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- E
  Eric WilliamsAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
M
Maria CaseyAug 22, 2025
What to do if adapters stick in Beckman Coulter buckets?
- A
  Angela WilsonAug 22, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
J
Jordan WareAug 26, 2025
How to prevent Beckman Coulter tube leakage?
- C
  Curtis RodriguezAug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
S
Shane JonesAug 27, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- T
  Tammy LynchAug 28, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
B
brittneysmithAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- C
  Crystal ThomasAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
L
Leslie HarrisSep 2, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- Y
  yorkericSep 2, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
S
Seth ChenSep 7, 2025
Why are my Beckman Coulter tubes collapsing?
- M
  Michelle PowellSep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
T
Todd MartinSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- R
  Robin HoffmanSep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
J
Jimmy CarlsonSep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- C
  Cassandra ThompsonSep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter J2-HC Specifications

General

Drive System	Direct Drive
Temperature Range	0 to 40°C
Dimensions	74 cm
Timer	Up to 99 hours, 59 minutes, HOLD
Control Panel	Digital Display
Weight	680 kg

Summary

Safety Notice

Safety Alerts and General Precautions

Explains safety alert symbols, chemical, biological, and mechanical safety guidelines.

CHAPTER 1: Rotors

Introduction and General Description

Rotor Materials and Design

Rotor Selection and Types

Centrifugation Techniques

Explains pelleting, isopycnic, and rate zonal separation methods.

General Operating Information

Covers rotor balance, tie-down, speed limits, and temperature compensation.

CHAPTER 2: Tubes, Bottles, and Accessories

Labware Selection Criteria

Labware Types and Materials

Special Labware Considerations

Filling, Sealing, and Sample Recovery

CHAPTER 4: Using Fixed-Angle Rotors

Operation, Installation, and Removal

Covers operating the centrifuge, installing, and removing fixed-angle rotors.

CHAPTER 5: Using Swinging-Bucket Rotors

Rotor Preparation and Loading

Details safety checks, preparation, and loading procedures for swinging-bucket rotors.

Symmetric and Balanced Loading

Explains principles for symmetric and balanced loading to ensure stability and performance.

Bucket and Carrier Loading Procedures

CHAPTER 6: Using Vertical-Tube and Rack-Type Rotors

Introduction and Description

Introduces vertical-tube and rack-type rotors and their basic characteristics.

Using Vertical-Tube Rotors

Using Rack-Type Rotors

CHAPTER 7: Care and Maintenance

Rotor Care Procedures

Covers cleaning, decontamination, sterilization, inspection, and lubrication of rotors.

Labware Care and Storage

Service and Troubleshooting

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