Beckman Coulter Avanti JXN 30 User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

D
Daniel SampsonAug 18, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- T
  Tanya DaltonAug 18, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
M
maurice30Aug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- M
  Mary ChungAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
A
Allen EdwardsAug 22, 2025
What to do if adapters stick in Beckman Coulter buckets?
- R
  Richard SanchezAug 23, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
R
rhenryAug 26, 2025
How to prevent Beckman Coulter tube leakage?
- T
  Todd SalasAug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
A
Alex HamiltonAug 27, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- R
  Robert HerringAug 28, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
D
Daniel GuzmanAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- A
  Alexander CarterAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
J
joshuamurraySep 2, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- R
  Ronald OwenSep 2, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
L
Leon CamachoSep 7, 2025
Why are my Beckman Coulter tubes collapsing?
- A
  amataSep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
B
Belinda WhiteSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- C
  christopher56Sep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
D
Dennis MontgomerySep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- T
  Thomas GeorgeSep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter Avanti JXN 30 Specifications

General

Max Speed	30, 000 RPM
Max RCF	100, 000 x g
Control Interface	Touchscreen
Drive System	Brushless DC motor
Capacity	4 x 1, 000 mL
Refrigeration System	CFC-free
Timer	Up to 99 hr, 59 min; Hold

Summary

Safety Notice

Chemical and Biological Safety

Discusses safety precautions for handling chemical and biological materials in centrifuges.

Mechanical Safety

Covers safe operating practices related to rotors and centrifuge mechanics.

CHAPTER 1 Rotors

Rotor Operation Principles

Covers pelleting, isopycnic, and rate zonal separation techniques.

Rotor Balance and Tie-Down

Details rotor balance and tie-down procedures for safe operation.

Overspeed Protection and Run Speeds

CHAPTER 2 Tubes, Bottles, and Accessories

Labware Selection Criteria

Tube and Bottle Types

Temperature Limits for Labware

Adapters and Canisters

CHAPTER 3 Tubes, Bottles, and Accessories

Gradient Preparation

Methods for preparing density gradients for centrifugation.

Filling and Sealing Tubes

Procedures for filling and sealing various types of tubes.

Capping Multiwell Plates

Sample Recovery

Procedures for recovering samples from tubes, cups, and Quick-Seal tubes.

CHAPTER 4 Using Fixed-Angle Rotors

Rotor Installation and Removal

Instructions for installing and removing fixed-angle rotors from the centrifuge.

CHAPTER 5 Using Swinging-Bucket Rotors

Rotor Preparation and Loading

Steps for preparing and loading swinging-bucket rotors.

Symmetric and Balanced Loading

Guidelines for symmetric and balanced loading of swinging-bucket rotors.

Bucket and Carrier Loading

CHAPTER 6 Using Vertical-Tube and Rack-Type Rotors

Vertical-Tube Rotor Usage

Rack-Type Rotor Usage

CHAPTER 7 Care and Maintenance

Rotor Care Fundamentals

Key aspects of rotor care including cleaning, inspection, and lubrication.

Cleaning and Decontamination

Sterilization and Inspection

Labware Care

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