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Avanti J-HC
Beckman Coulter Avanti J-HC User Manual
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PN JR-IM-10AG
October 2014
Instructions F
or Use
Rotors and T
ubes
F
or Beckman Coulter
J2, J6, and A
vanti J Series Centrifuges
Beckman Coulter, Inc.
250 S. Kraem
er Blvd.
Brea, CA 92821 U.S.A.
2
Table of Contents
Revision History
3
Safety Notice
5
Alerts for Warning, Caution, Important, and Note
5
Chemical and Biological Safety
5
Mechanical Safety
6
Rohs Notice
7
Table of Contents
9
Rotors
19
Introduction
19
General Description
19
Rotor Designations
19
Fixed-Angle, Swinging-Bucket, Vertical-Tube, and Rack-Type Rotors
20
Materials
21
Drive Pins
22
Rotor Selection
22
Rotors Used in Beckman Coulter J Series Centrifuges
23
Particle Separation in Fixed-Angle, Swinging-Bucket, and Verti
28
Pelleting (Differential Separation)
29
Sedimentation Coefficients (in Svedberg Units) for some Com
30
Nomogram for J2 Series Centrifuges
31
Nomogram for J6Series Centrifuges
32
Isopycnic Separations
33
Rate Zonal Separations
33
Blood Component Separations
34
General Operating Information
34
Rotor Balance
34
Bucket Rotors
34
Rotor Tie-Down
35
Arranging Tubes Symmetrically in a Fixed-Angle, Vertical-Tube, or JS-24 Series Swinging-Bucket Rotor
35
Overspeed Protection
36
Allowable Run Speeds
36
Temperature Compensation
37
Avanti J Centrifuges
37
Avanti J2 Series Centrifuges (no Longer Manufactured)
38
Analog J6 Series Centrifuges (no Longer Manufactured)
38
Microprocessor-Controlled Centrifuges
39
Tubes, Bottles, and Accessories
41
Introduction
41
Labware Selection Criteria
41
Labware Material Compatibility with Solvents and Sample
42
Characteristics and Chemical Resistances of Tube and Bottle
42
Gradient Formation and Fractionation
43
Labware Types
43
Polypropylene Tubes
43
Open-Top Polypropylene Tubes
43
Quick-Seal Polypropylene Tubes
44
Polycarbonate Tubes
44
Polyethylene Tubes
44
Ultra-Clear Tubes
44
Stainless Steel Tubes
45
Microfuge Tubes
45
Bottles
45
Multiwell Titer Plates
45
96-Well Microtiter Plates
45
Deep-Well Titer Plates (and Caps)
46
Square-Well Titer Plates
46
Temperature Limits
46
Spacers and Floating Spacers
47
Adapters
47
Bottle Adapters
47
Multitube Adapters
47
Solid Multitube Adapters
48
Modular Disk Adapters
48
Bottle and Tube Caps
49
Aerosolve Canisters
49
Blood Bag Cups
50
Rotor Labware Assemblies
50
Tubes, Bottles, and Accessories
51
Introduction
51
Gradient Preparation
51
General Filling and Sealing or Capping Requirements
52
General Filling and Sealing Requirements for Tubes and
53
Working with Physiological Fluids
54
Filling Open-Top Tubes
55
Open-Top Polypropylene Tubes
55
Swinging-Bucket Rotors
55
Fixed-Angle Rotors
55
Other Open-Top Tubes
55
Polycarbonate
55
Ultra-Clear
55
Polypropylene
56
Polyethylene
56
Stainless Steel
56
Capping Tubes
56
Filling and Capping Tubes
57
Three-Piece Assemblies
57
JLA-8.1000 and JLA-9.1000 Bottle Cap/Closure
57
Filling and Loading Cups in the
58
Rotor
58
Filling and Sealing Quick-Seal Tubes
59
The Cordless Quick-Seal Tube Topper
60
Capping Multiwell Titer Plates
64
Cap Strips
64
Aluminum Foil Lids
64
Using Adapters
64
Using Solid Multitube Adapters
64
Using Modular Disk Multitube Adapters
65
Using Aerosolve Canisters
66
Using Canisters as Wide-Mouth Bottles
67
Using Canisters with Tube Racks
68
Aerosolve Tube Rack
68
Using Blood Bag Cups
69
Sample Recovery
70
Capped Tubes
71
Cups
71
Quick-Seal Tubes
72
Making Ultra-Clear Tubes Wettable
73
Using Fixed-Angle Rotors
75
Introduction
75
Description
75
Examples of Fixed-Angle Rotors
76
General Specifications for Beckman Coulter J Series Fixed-Angle Rotors
77
Tubes and Bottles
80
Rotor Preparation and Loading
81
Prerun Safety Check
81
Rotor Preparation
81
Operation
82
Installing the Rotor
83
Removal and Sample Recovery
84
Using Swinging-Bucket Rotors
87
Introduction
87
Description
87
Examples of Swinging-Bucket Rotors
88
General Specifications for Beckman Coulter J Series Swinging
89
Labware
90
Rotor Preparation and Loading
91
Prerun Safety Check
91
Rotor Preparation
91
Special Preparation Instructions for JS-24 Series Rotors
92
Loading the Rotor Yoke
93
Loading JS-24 Series Rotors
94
Symmetric and Balanced Loading
95
Examples of Correctly and Incorrectly Loaded Buckets and Carriers
96
Loading Buckets
97
Using Modular Disk Adapters
97
Using Blood Bag Cups
98
Loading Buckets into the Rotor
99
Typical Blood Bag Loading Procedures
99
Using Microtiter Plate Carriers
100
Micro Plus Carriers
100
JS-5.9 and JS-5.3 Plate Carriers
101
The Micro Plus Microtiter Plate Carrier, Base, Pad, and Deep
101
J6 Carriers
102
Microplate Carriers Used with J6-Series Rotors
102
Operation
103
Sample Recovery
103
Removing JS-24 Series Rotors
103
And Rack-Type Rotors,6-1
105
Using Vertical-Tube and Rack-Type Rotors
105
Introduction
105
Description
105
General Specifications for Beckman Coulter J Series Vertical-Tube and Rack-Type Rotors
105
Vertical-Tube Rotors
106
Rack-Type Rotors
106
Vertical-Tube Rotor
106
Rack-Type Rotor
106
Using a Vertical-Tube Rotor
107
Tubes and Bottles
107
Rotor Preparation and Loading
107
Prerun Safety Checks
107
Rotor Preparation
107
Operation
108
Installing the Rotor
109
Removal and Sample Recovery
110
Using a Rack-Type Rotor
111
Trays and Tubes
111
Rotor Preparation and Loading
111
Prerun Safety Checks
111
Rotor Preparation
111
Operation
112
Installing the Rotor
113
Removal and Sample Recovery
114
Care and Maintenance
115
Introduction
115
Rotor Care
115
Cleaning
116
Decontamination
117
Sterilization and Disinfection
118
Inspection
118
Lubrication
119
O-Rings
119
Pivot Pins and Buckets
119
Field Rotor Inspection Program
120
Tube, Bottle, and Accessory Care
120
Cleaning
120
Decontamination
121
Sterilization and Disinfection
121
Tube and Bottle Sterilization and Disinfection
122
Inspection
123
Tube and Bottle Storage
123
Returning a Rotor or Accessory to the Factory
124
Diagnostic Hints
124
Troubleshooting Chart
125
Products,A-1
129
Chemical Resistances for Beckman Coulter Centrifugation Products
129
List of Chemical Resistances
129
Temperature Compensation Tables
131
Introduction
131
Temperature Compensation
131
Temperature Compensation Settings for Thej2-HC Centrifuge
131
Temperature Compensation Settings for the J2-21, J2-21B
133
Temperature Compensation Settings for the J2-MI, J2-21M, J2-MC, and J2-21M/E Centrifuges
135
Temperature Compensation Settings for the J6
136
Gradient Materials
137
Introduction
137
Materials
138
Commonly Used Gradient Materials with Their Solvents
138
Density, Refractive Index, and Concentration Data-Cesium
139
Density, Refractive Index, and Concentration Data-Sucrose at
140
Blood Component Separation
143
Introduction
143
Blood Bank Collection Overview
143
Components and Typical Usage
144
Single-Donor Fresh Plasma
144
Single-Donor Plasma
144
Packed Red Blood Cells
144
Leukocyte-Depleted Red Blood Cells
144
Shelf Life
145
Freezing
145
Separation of Blood Components by Centrifugation
145
Blood Component Storage
145
Blood Component Separation
146
Blood Bank Methods
147
Tips for Optimum Centrifugation Runs
148
References
149
List of References
149
4
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Beckman Coulter Avanti J-HC Specifications
General
Brand
Beckman Coulter
Model
Avanti J-HC
Category
Laboratory Equipment
Language
English
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