Why are my Beckman Coulter tubes cracking?
Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
Why are my Beckman Coulter tubes collapsing?
Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
What to do if Beckman Coulter tubes with snap-on caps are leaking?
If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
What to do if Beckman Coulter Quick-Seal tubes are leaking?
If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
How to prevent Beckman Coulter tube leakage?
To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
What to do if adapters stick in Beckman Coulter buckets?
To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
What to do if my Beckman Coulter rotor lid is hard to remove?
If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
What to do for a momentary power failure on Beckman Coulter Avanti J-HC Laboratory Equipment?
General| Vacuum System | Not applicable |
|---|---|
| Type | High-capacity centrifuge |
| Temperature Range | -10°C to 40°C |
| Refrigeration System | CFC-free |
| Timer | Up to 99 hours, 59 minutes |
| Weight | 875 lb (397 kg) |
Guide to choosing the appropriate rotor for specific applications and centrifuges.
Essential operational procedures including rotor balance and tie-down.
Factors to consider when choosing tubes, bottles, and accessories for applications.
Overview of different types of labware available for centrifugation.
Guidelines for filling and sealing labware to ensure sample integrity and prevent leakage.
Methods for retrieving separated components from labware after centrifugation.
Procedures for preparing and loading swinging-bucket rotors.
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