Beckman Coulter J2-HS User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

E
Emily MarshallAug 18, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- K
  Katherine FullerAug 18, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
C
Casey ParkerAug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- S
  Sherri ValentineAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
M
Mrs. Amanda MontgomeryAug 23, 2025
What to do if adapters stick in Beckman Coulter buckets?
- J
  Joanne HaynesAug 23, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
M
millerlisaAug 26, 2025
How to prevent Beckman Coulter tube leakage?
- D
  Daniel JohnsonAug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
C
Cynthia MartinAug 28, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- C
  Craig FitzpatrickAug 28, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
W
wrightstevenAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- E
  Eric RuizAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
K
karen98Sep 2, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- S
  sharon29Sep 2, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
J
John RobertsonSep 7, 2025
Why are my Beckman Coulter tubes collapsing?
- L
  Laura RoySep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
J
James TurnerSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- H
  heather09Sep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
L
Leonard AllenSep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- S
  sallygrantSep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter J2-HS Specifications

General

Maximum Speed	21, 000 RPM
Temperature Range	0 to 40 °C
Capacity	6 x 250 mL
Power Requirements	50/60 Hz
Dimensions	71 cm x 84 cm x 91 cm
Weight	475 lb (215 kg)

Summary

Safety Notice

Chemical and Biological Safety

Discusses safety procedures for handling hazardous materials.

Mechanical Safety

Covers safety guidelines for mechanical aspects of rotor and centrifuge operation.

Care and Maintenance

Rotor Care

Outlines essential aspects of rotor care and maintenance.

Decontamination

Details procedures for decontaminating rotors exposed to hazardous materials.

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