Beckman Coulter J6-HC User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

J
Jeffrey WileyAug 17, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- S
  Sabrina RobinsonAug 17, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
G
Glen JohnsonAug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- B
  Brett HuangAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
M
Mary HopkinsAug 22, 2025
What to do if adapters stick in Beckman Coulter buckets?
- A
  Amber BrownAug 22, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
E
Edward NunezAug 25, 2025
How to prevent Beckman Coulter tube leakage?
- V
  Vanessa LongAug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
G
George KingAug 27, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- K
  Karen CantuAug 27, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
K
Katherine HernandezAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- E
  Erin WilliamsAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
L
Lisa RodriguezSep 1, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- M
  melissa47Sep 1, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
W
William CollinsSep 6, 2025
Why are my Beckman Coulter tubes collapsing?
- C
  Carol BlackSep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
J
Jaclyn MelendezSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- K
  Kevin AlvaradoSep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
S
Susan GillSep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- V
  vmurphySep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter J6-HC Specifications

General

Maximum Speed	6, 000 RPM
Refrigeration System	CFC-free
Frequency	50/60 Hz
Capacity	6 liters
Drive	Direct
Timer	0-99 minutes
Voltage	208-240V
Type	Floor centrifuge

Summary

Rotors

Rotor Selection and Usage

Guidance on choosing rotors, general operation, balance, and tie-down.

Separation Techniques

Operating Parameters

Tubes, Bottles, and Accessories

Introduction and Selection Criteria

Labware Types and Specifications

General Filling and Sealing or Capping Requirements

Guidelines on fill levels and sealing methods for various tubes and bottles.

Working with Physiological Fluids

Safety precautions and containment recommendations for handling hazardous fluids.

Filling and Sealing Quick-Seal Tubes

Sample Recovery

Techniques for safely recovering samples from tubes and rotors.

Using Fixed-Angle Rotors

Preparation, Loading, and Operation

Steps for rotor preparation, loading, operation, installation, and removal.

Using Swinging-Bucket Rotors

Preparation, Loading, Operation, and Recovery

Procedures for rotor preparation, loading, operation, and sample recovery.

Removing JS-24 Series Rotors

Using Vertical-Tube Rotors

Using Rack-Type Rotors

Rotor Preparation, Operation, and Recovery

Steps for rotor preparation, operation, installation, and sample recovery.

Care and Maintenance

Rotor Care

Guidelines for cleaning, decontamination, sterilization, inspection, and lubrication of rotors.

Tube, Bottle, and Accessory Care

Guidelines for cleaning, sterilization, and inspection of tubes, bottles, and accessories.

Equipment Return and Troubleshooting

Temperature Compensation Tables

Temperature Compensation

Explanation of why temperature compensation is needed for rotors.

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