Beckman Coulter J6-MI User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

M
Michael SanchezAug 17, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- T
  Travis TuckerAug 18, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
L
lawrencetroyAug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- C
  Chelsea GloverAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
C
Carlos CombsAug 22, 2025
What to do if adapters stick in Beckman Coulter buckets?
- H
  harrisonjacobAug 22, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
E
Eric DelgadoAug 25, 2025
How to prevent Beckman Coulter tube leakage?
- H
  haleyaustinAug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
M
Megan BradfordAug 27, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- J
  Jessica FosterAug 27, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
E
Edwin PalmerAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- J
  Joel CopelandAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
C
Craig ThompsonSep 1, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- J
  James PattersonSep 2, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
M
melissafloresSep 6, 2025
Why are my Beckman Coulter tubes collapsing?
- B
  bhatfieldSep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
C
christinerobinsonSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- D
  Denise GomezSep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
C
Christine StokesSep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- W
  wellstylerSep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter J6-MI Specifications

General

Brand	Beckman Coulter
Model	J6-MI
Category	Laboratory Equipment
Language	English

Summary

Safety Notice

Alerts for Warning, Caution, Important, and Note

Explanation of warning symbols, terms, and their significance.

Chemical and Biological Safety

Precautions for handling hazardous chemical and biological materials.

Mechanical Safety

Safety guidelines related to mechanical aspects of centrifuge operation.

Chapter 1 Rotors

Separation Principles

General Operating Information

Chapter 2 Tubes, Bottles, and Accessories

Labware Types

Chapter 3 Tubes, Bottles, and Accessories

General Filling and Sealing or Capping Requirements

Working with Physiological Fluids

Safety precautions for handling biological samples.

Specific Filling and Sealing

Chapter 4 Using Fixed-Angle Rotors

Operation

Installation and Removal

Procedures for installing and removing rotors.

Chapter 5 Using Swinging-Bucket Rotors

Preparation and Loading

Steps for preparing and loading swinging-bucket rotors.

Symmetric and Balanced Loading

Critical procedures for stable rotor operation.

Operation and Sample Recovery

Instructions for running and retrieving samples.

Chapter 6 Using Vertical-Tube and Rack-Type Rotors

Vertical-Tube Rotor Usage

Rack-Type Rotor Usage

Chapter 7 Care and Maintenance

Cleaning, Decontamination, and Sterilization

Procedures for cleaning, decontaminating, and sterilizing rotors.

Inspection and Lubrication

Regular checks and lubrication of rotor parts.

Troubleshooting and Service

Diagnostic hints and procedures for returning items for service.

Appendix A Chemical Resistances for Beckman Coulter Centrifugation Products

List of Chemical Resistances

Appendix B Temperature Compensation Tables

Compensation Settings Tables

Appendix D Blood Component Separation

Centrifugation for Separation

Optimum Centrifugation Tips

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