Beckman Coulter J6 series User Manual

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PN JR-IM-10AG

October 2014

Instructions For Use

Rotors and Tubes

For Beckman Coulter

J2, J6, and Avanti J Series Centrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Table of Contents

Questions and Answers:

N
Natalie WyattAug 17, 2025
What to do if my Beckman Coulter rotor lid is hard to remove?
- P
  Peter MorseAug 18, 2025
  If you're having trouble removing the rotor lid, canister cover, or bucket cover from your Beckman Coulter equipment after centrifugation, it could be due to a vacuum buildup inside the container. Try lifting the vent plug on buckets or canister covers with vents to relieve the vacuum. Alternatively, the threads may be contaminated with dirt, dried lubricant, or metal particles, or they may be insufficiently lubricated. Routinely clean metal threads with a diluted solution of Solution 555 (10 parts water to 1 part detergent), and then lubricate them with Spinkote.
V
Victor KellyAug 20, 2025
Why is my Beckman Coulter bottle leaking (bottles with cap assemblies)?
- M
  Mary ButlerAug 20, 2025
  Leakage in bottles with cap assemblies in Beckman Coulter equipment can occur due to: * Moisture or lubrication on the cap or sealing surface. Ensure the O-ring, plug, and bottle lip are dry and free of lubrication before use. * A damaged or defective O-ring or gasket. Replace the O-ring or gasket. * Insufficiently tightened cap. Tighten the cap securely. * An uneven sealing surface on the bottle. Replace the bottle. * Overfilled tube. If using threaded caps without inserts or O-rings, keep the meniscus lower to prevent leakage.
M
Mary OrtegaAug 23, 2025
What to do if adapters stick in Beckman Coulter buckets?
- T
  Timothy BurkeAug 23, 2025
  To prevent adapters from sticking in buckets after centrifugation in your Beckman Coulter equipment, apply a thin film of powder, such as talcum powder, to the tube adapter rubber bases after cleaning or as required.
M
Mrs. Shelly WhitakerAug 26, 2025
How to prevent Beckman Coulter tube leakage?
- R
  robert53Aug 26, 2025
  To prevent tube leakage in your Beckman Coulter equipment, ensure that caps are properly seated on tubes and fully tightened. Also, make sure all cap components are thoroughly dry before assembly.
D
David LoganAug 28, 2025
What to do if Beckman Coulter Quick-Seal tubes are leaking?
- L
  Laura MartinezAug 28, 2025
  If Quick-Seal tubes are leaking in your Beckman Coulter equipment, it's likely due to improper sealing. After heat-sealing, gently squeeze the tube to test the seal for leaks. If the tube leaks, reseal it.
T
Teresa EdwardsAug 30, 2025
Why is my Beckman Coulter bottle leaking (uncapped bottles)?
- R
  Rachel GrantAug 30, 2025
  If an uncapped bottle is leaking in your Beckman Coulter equipment, it is likely because the bottle is too full. The meniscus must be kept lower to prevent leakage.
L
lwilsonSep 1, 2025
What to do if Beckman Coulter tubes with snap-on caps are leaking?
- H
  Hector FernandezSep 1, 2025
  If tubes with snap-on caps are leaking in your Beckman Coulter equipment, ensure the tube is not too full; the meniscus must be kept lower to prevent leakage.
B
Bethany BriggsSep 6, 2025
Why are my Beckman Coulter tubes collapsing?
- S
  Shannon BlankenshipSep 7, 2025
  Tube collapse in Beckman Coulter equipment can occur due to several reasons: * Insufficient thinwall tube volume: The meniscus should be 2 to 3 mm below the tube top to provide adequate tube wall support. * Moisture: Ensure that tubes and tube cavities or buckets are dry before inserting the tubes, as moisture can cause the tube to float and collapse. * Reagent incompatibility: Refer to Chemical Resistances for chemical compatibilities of the tube material and chemicals used. * Excessive speed: Refer to the applicable rotor manual for maximum speeds to ensure tubes are not run above their rated speed.
E
Elizabeth HicksSep 9, 2025
Why are my Beckman Coulter tubes cracking?
- M
  Maria RogersSep 9, 2025
  Cracking in Beckman Coulter tubes can be attributed to: * Low temperature: Tubes may crack or become brittle if used below their lower temperature limit. Before using tubes at other than stated temperature limits, evaluate them under centrifugation conditions. * Aging: Tubes may become brittle with age and use. Dispose of brittle or cracked tubes.
T
Tina WalkerSep 14, 2025
What is the reason for leakage of uncapped tubes in Beckman Coulter equipment?
- L
  Leslie HicksSep 14, 2025
  If uncapped tubes are leaking in your Beckman Coulter equipment, it's likely because the tube volume exceeds the maximum uncapped volume.

Beckman Coulter J6 series Specifications

General

Model Series	J6 Series
Type	Centrifuge
Maximum Speed	6000 rpm
Timer	0-99 minutes
Capacity	6 x 1 liter bottles
Power Requirements	50/60 Hz

Summary

Safety Notice

Alerts for Warning, Caution, Important, and Note

Explains safety alert symbols and their meanings for safe operation.

Chemical and Biological Safety

Guidelines for handling hazardous materials and biological samples safely.

Mechanical Safety

Safety precautions related to rotor and centrifuge mechanical operation.

Rotors

Rotor Selection

Guides the selection of appropriate rotors for centrifugation applications.

General Operating Information

Provides essential information for operating centrifuges and rotors safely.

Tubes, Bottles, and Accessories

Labware Selection Criteria

Factors to consider when choosing labware for centrifugation.

Labware Types

Overview of different types of tubes, bottles, and accessories.

Using Fixed-Angle Rotors

Rotor Preparation and Loading

Steps for preparing and loading fixed-angle rotors for operation.

Operation

Procedures for operating fixed-angle rotors.

Using Swinging-Bucket Rotors

Rotor Preparation and Loading

Steps for preparing and loading swinging-bucket rotors.

Operation

Procedures for operating swinging-bucket rotors.

Using Vertical-Tube and Rack-Type Rotors

Rotor Preparation and Loading

Steps for preparing and loading vertical-tube rotors.

Operation

Procedures for operating vertical-tube rotors.

Care and Maintenance

Rotor Care

Guidelines for maintaining rotors in optimal condition.

Cleaning

Procedures for cleaning rotors and components.

Inspection

Guidelines for inspecting rotors and components for damage.

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