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Eppendorf 5424 - Centrifuging

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Operation
Centrifuge 5424/5424 R
English (EN)
30
During continuous cooling the following applies:
The target temperature is displayed.
Irrespective of the target temperature, continuous cooling does not go below 4°C to prevent the rotor
chamber and the samples from freezing and increased condensation in the device.
The temperature adjustment takes longer because the rotor is not rotating.
Open the centrifuge lid to end continuous cooling.
If the centrifuge is not used for more than 8 hours, the continuous cooling is switched off automatically.
This protects against ice formation in the rotor chamber and the tubes, and against increased condensation
in the device.
The device then switches to standby mode. The EP logo appears in the display.
With fast temp you can quickly reach the desired temperature again (see p. 29).
You can change continuous cooling to endless operation. To do so, in the device menu under TEMP enable
the 'oo' (see p. 25) option. Note that this may reduce the service life of the compressor.
5.6 Centrifuging
Before using the Centrifuge 5424/5424 R for the first time, familiarize yourself with the operating controls
and the display (see Overview of operating controls on p. 23).
Each of the centrifuging variants described here must be preceded by the preparation described above (see
Preparing for centrifugation on p. 26).
Only 5424 R: Please also note the instructions on cooling (see p. 28).
CAUTION! Risk from incorrectly-loaded rotors and damaged/overloaded tubes!
Before commencing centrifugation, follow the safety instructions relating to risks from
asymmetrically loaded and/or overloaded rotors and from overloaded, damaged and/or
open tubes (see Warnings for intended use on p. 9).
WARNING! Risk of injury from improperly attached rotors and rotor lids.
Only centrifuge with rotor and rotor lid firmly tightened.
If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the key to stop centrifuging.

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