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Ensure correct power supply and grounded socket usage for device safety.
Avoid explosive materials, liquids, and ensure proper ventilation for safe operation.
Caution about hot surfaces and use of temperature-stable materials.
Understanding warning symbols for thermoblock and gradient mode.
List of items included in the Mastercycler ® delivery package.
Guidelines for placing the device and ensuring adequate ventilation.
Steps to connect power and prepare the device for its first use.
Description of the physical components and views of the Mastercycler ®.
Explanation of the programming and cursor keys on the control panel.
Detailed functions of specific programming keys for operation and editing.
Usage of cursor keys for navigation and control keys for menu/program interaction.
Understanding the menu and programming fields of the device display.
Overview of the main menu and methods for navigating through its options.
Procedures for initiating program execution, either directly or from a list.
Accessing and managing program files (Edit, Load, Standard, New, Delete).
How to modify programs already stored in the device or on a card.
Retrieving programs from internal memory or personal cards for editing.
Utilizing a pre-defined program as a template for new programs.
Steps to initiate and define a completely new PCR program sequence.
Removing unwanted programs from the device's internal memory.
Configuring general system parameters like time, date, and printer settings.
Customizing the program editor view, including displaying extended temperature options.
Configuring printout editor and print protocol settings for printer output.
Utilizing the gradient feature for temperature distribution across the thermoblock.
Configuring device time, PC connection rates, and audible feedback.
Setting device startup behavior and automatic program restart after power loss.
Using specific programs for device testing, validation, and temperature calibration.
Adjusting heated lid temperature and on/off status for condensation prevention.
Setting block temperature for incubation or preheating, aligning lid temperature.
Guidelines for preparing the thermoblock and loading various tube types or plates.
Setting sample volume and adapting temperature control to tube type.
Details on heated lid function, condensation prevention, and burn risks.
Procedures for preheating the thermoblock for incubation or faster start times.
Steps to switch on the device and view the initial display.
Initiating program execution, either directly or from a list.
Entering tube type and fill volume for programs using the CNTRL/TUBE command.
Viewing approximate run time and expected end time of an active program.
Procedures for pausing or interrupting an ongoing PCR program.
Steps to continue a program that was previously paused or interrupted.
How to completely cancel an active PCR program.
Steps for safely switching off the Mastercycler ® device.
Introduction to program structure, line limits, and command entry methods.
Defining block temperature regulation mode (Block or Tube).
Setting heated lid temperature and its behavior at program start/end.
Entering target temperatures, increments, and cycle times for program steps.
Adjusting temperature and time per cycle for program steps.
Defining heating/cooling rates and their incremental changes per cycle.
Setting temperature gradients across the block for Mastercycler ® gradient models.
Pausing program execution while maintaining a specific temperature.
Temporarily interrupting a program, allowing for user-defined text entry.
Repeating program sections or entire programs for multiple cycles.
Activating acoustic signals for program events like pauses or completion.
Connecting multiple programs to run sequentially.
Finalizing a program and switching off temperature control.
General process for creating new PCR programs, including gradient options.
Detailed instructions for entering commands like CNTRL, LID, T, and time.
Procedures for saving a newly created or modified program with a specific name.
Loading and editing programs already stored in memory or on a personal card.
Detailed steps for changing program lines, inserting, deleting, and saving modifications.
Utilizing the "Standard" example program as a base for new program creation.
Steps to remove programs from the device's memory.
Introduction to various programming examples demonstrating device versatility.
Specific programming steps for performing Gradient PCR on compatible models.
Example program demonstrating Hot Start PCR technique with pause for reagent addition.
Example program illustrating Touch Down PCR with temperature increments per cycle.
Example program for Long PCR, focusing on extending elongation time per cycle.
Example program for RAPD-PCR, detailing heating rates and gradual temperature changes.
Advanced programming technique for keeping samples cool before a linked PCR protocol.
Quick steps for powering up the Mastercycler ® and accessing the main menu.
Instructions for editing the pre-defined "Standard" program for custom use.
Brief steps for saving programs and managing lines (adding/deleting).
Summarized access to system settings and program execution controls (start, stop, interrupt).
Guidelines for safe handling, insertion, removal, and formatting of the personal card.
Steps for managing programs (start, load, save, delete) using the personal card.
Connecting the device to printers and PCs via specified ports and cables.
Illustrative examples of the data format generated during program printout and protocol.
Configuring PC communication settings and transferring programs between devices.
Listing common error messages, their causes, and initial solutions.
Step-by-step instructions for running the built-in test program for device monitoring.
Catalog of disposable items and recommended accessory devices for the Mastercycler ®.
Listing of available PCR reagents, including enzymes and nucleotide mixes.
Contact details for Eppendorf North America, Inc. for support, repair, and lab inquiries.
Contact details for Brinkmann Instruments (Canada) Ltd. for support, repair, and lab inquiries.
General| Block format | 96-well |
|---|---|
| Capacity | 96 x 0.2 ml tubes |
| Temperature uniformity | ±0.3°C |
| Display | LCD |
| Interface | RS-232 |
| Power requirements | 50/60 Hz |
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