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Eppendorf Mastercycler Gradient - Creating Programs Using Example; Deleting Programs

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35
7 Programming
7.4 Creating programs using an example program
It is possible to program quickly with the aid of the "Standard" example program. A program does not need to
be completely rewritten; the sample can be supplemented, modified and then saved under a new name.
Select the "FILES" menu and call up by pressing .
Select "Standard" and call up by pressing .
The example program is loaded into the processing level. It is structured in the following way:
Although the example program cannot be deleted, individual changes can be made. After changes have been
made, the example program must be saved under the name "Standard". To restore the original example
program, the "Standard" program in the "Delete" sub-menu must be deleted. When "Standard" ís called up,
the original example program is reloaded into the processing level.
7.5 Deleting a program
Select the "FILES" menu and call up by pressing .
Select the program name.
Confirm by pressing . The program is deleted.
The procedure for deleting programs on a personal card is described in Sec. 9.3.
CNTRL BLOCK Temperature control for the block.
LID=105° Sets heated lid to 105 °C.
NOWAIT AUTO NOWAIT = Program is started immediately.
AUTO = Lid heating is switched off automatically
when temperature (22 °C) is maintained
for more than 5 minutes.
1 T=94.0° 0:02:00 Maintains 94 °C for 2 min. (initial denaturation)
2 T=94.0° 0:00:15 Maintains 94 °C for 15 secs. (denaturation)
3 T=44.0° 0:00:15 Maintains 44 °C for 15 secs. (annealing)
4 T=72.0° 0:00:30 Maintains 72 °C for 30 secs. (elongation)
5 GOTO 2 REP 24 Repeats 24 times from program step 2 onwards.
The total number of cycles is 25.
6 T=72.0° 0:00:30 Maintains 72 °C for 30 secs. (final elongation)
7 HOLD 22.0° ENTER Cools down to 22 °C and maintains temperature.
end Program end
Select "Delete" and call up by
pressing .
A list of the existing programs
appears in the display.
FILES . . Delete Program
Edit TEST 1
Load TEST 2
Standard
New
Delete
STANDARD
– –/22.0° 10:22:06

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