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Eppendorf Mastercycler nexus - User Manual

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Mastercycler
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nexus
Operating manual

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Summary

Operating Instructions

Using This Manual

Guidance on how to read and use the operating manual effectively.

Danger Symbols and Levels

Explanation of hazard symbols and their corresponding danger levels.

Symbols Used

Explanation of the various symbols used throughout the manual.

Abbreviations Used

List of abbreviations and their meanings used in the manual.

Glossary

Definitions of key terms and concepts used in the manual.

Product Description

Main Illustration and Variants

Overview of the instrument's front and rear views and its different variants.

Terminal Block and Control Panel

Description of the terminal block and the control panel interface.

Delivery Package Contents

List of items included in the Mastercycler nexus delivery package.

Product Features

Detailed explanation of key technologies and functionalities of the Mastercycler nexus.

Control Panel and Ports

Description of the control panel and available ports on different models.

Consumables

Information on compatible consumables for the Mastercycler nexus.

Networking and Service

Details on connecting the device to a network and available service options.

Safety

Intended Use

Specifies the intended applications and laboratory environment for the device.

User Profile

Information on who is authorized to operate the device.

Product Liability Information

Conditions under which Eppendorf's liability is voided.

General Warnings for Intended Use

Key safety precautions regarding potential hazards like explosion, fire, and biohazards.

Warning Signs on the Device

Explanation of specific warning symbols and their meanings displayed on the device.

Installation

Selecting the Location

Criteria for choosing a safe and stable location for the instrument.

Installing the Instrument

Steps for connecting the device to power and attaching multiple units.

Shut Down

Procedure for properly shutting down the Mastercycler nexus.

Operation

Initial Steps and Flexlid Heated Lid

Checking installation and instructions for using the flexlid heated lid.

Starting the Mastercycler Nexus

First-time startup procedure, including administrator PIN definition.

Logging In and Out

Procedures for logging in, changing users, and logging out.

Software Operation Overview

Explanation of the navigation tree and software interface elements.

Managing Folders and Programs

Creating, copying, and deleting folders and programs.

Using the Schedule

How to use the scheduling function for device reservation.

Power Save Mode

Instructions on how to activate and exit the device's power saving mode.

Programming

Overview of the Program Editor

Introduction to the graphic program editor and how to open it.

Making General Settings

Configuring program header settings like lid temperature and temp. mode.

Editing Programs

Detailed steps for inserting, editing, and deleting program steps.

Program Templates

Overview of available program templates for creating new programs.

PCR Run

Loading the Thermoblock

Selecting and inserting appropriate sample tubes and plates into the thermoblock.

Starting a Program

Procedure for selecting and starting a PCR program on the device.

Interrupting or Stopping Programs

How to pause, resume, or abort a running PCR program.

Displaying Recently Run Programs

Accessing a list of the most recently executed programs.

System Management

Administrator Functions

Overview of administrator privileges and access rights compared to default users.

Managing User Accounts

Creating, editing, and deleting user accounts and managing PINs.

System Settings

Configuration of system parameters like beeper, country settings, date/time, and PIN.

Cycler Functions

Viewing and adjusting thermoblock/lid status and accessing protocols.

Managing and Backing Up Data

Procedures for backing up and restoring device data.

Transferring Programs

Methods for transferring programs from older Mastercycler models.

Network Connection

Steps for setting up an Ethernet network connection for e-mail and log files.

Quick Start

Login Procedure

Step-by-step guide for logging into the Mastercycler nexus.

Creating Folders and Programs

Instructions for creating new folders and programs.

Copying Folders and Programs

Process for copying existing folders and programs.

Deleting Folders and Programs

How to remove folders and programs from the device.

Editing Programs

Guide on how to modify existing PCR programs.

Stopping and Starting Programs

Steps to start, stop, abort, or resume PCR programs.

Maintenance

Cleaning Procedures

Instructions for cleaning the device housing and thermoblock.

Disinfection and Decontamination

Methods for disinfecting and decontaminating the instrument.

Decontamination Before Shipment

Procedure for decontaminating the device prior to shipping.

Troubleshooting

General Errors

Basic troubleshooting steps for common device errors.

Transport, Storage and Disposal

Packing Guidelines

Instructions for packing the device for transport or storage.

Disposal Information

Regulations and guidelines for the proper disposal of the device.

Technical Data

Power Supply Specifications

Electrical requirements and power consumption details.

Weight and Dimensions

Physical specifications including weight and size of the instrument.

Ambient Conditions

Recommended operating and storage environmental conditions.

Application Parameters

Key parameters for various Mastercycler nexus models and their applications.

Ordering Information

Mastercycler Nexus Variants

Order numbers for different Mastercycler nexus master variants.

Eco Variants

Order numbers for different Mastercycler nexus eco variants.

Accessories

List of available accessories for the Mastercycler nexus.

Consumables

Information on compatible consumables like PCR plates and tubes.

Recommended Auxiliary Materials

List of recommended auxiliary materials for use with the instrument.

Eppendorf Mastercycler nexus Specifications

General IconGeneral
Max. Heating Rate3 °C/s
Max. Cooling Rate2 °C/s
DisplayLCD
Power Requirements100-240 V, 50/60 Hz
Temperature Uniformity±0.3°C
Block Formats96-well, 384-well
ConnectivityUSB, Ethernet

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