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Eppendorf Reference 2 - User Manual

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Eppendorf Reference
®
2
Operating manual

Table of Contents

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Summary

Operating instructions

Using this manual

Provides guidance on how to effectively use the operating manual.

Danger symbols and danger levels

Explains the meaning of safety symbols and danger levels used throughout the manual.

Symbols used

Defines various symbols and their corresponding meanings for clarity.

Safety

Intended use

Specifies the designated purpose and application scope of the Reference 2 pipette.

Warnings for intended use

Highlights critical safety warnings and precautions for proper operation.

Product description

Features

Details the main characteristics and functionalities of the Eppendorf Reference 2 pipette.

Delivery package

Lists all items included in the standard product delivery package.

Product overview

Provides a visual guide identifying key components of the pipette.

Materials

Outlines the materials used in the construction of the pipette's components.

Operation

Setting the volume

Step-by-step instructions for accurately setting the desired volume on the pipette.

Attaching the pipette tips

Guide on the correct procedure for attaching pipette tips.

Optimum immersion depths

Recommends optimal immersion depths for consistent and accurate liquid aspiration.

Forward pipetting

Detailed steps for performing the standard forward pipetting technique.

Reverse pipetting

Instructions for utilizing the reverse pipetting method for specific liquid types.

Storing the pipette

Guidance on appropriate methods for storing the pipette when not in use.

Troubleshooting

Control button

Solutions for common issues encountered with the pipette's control button.

Dispensing

Troubleshooting common problems encountered during the liquid dispensing process.

Pipette tip

Solutions for issues related to pipette tip fit and compatibility.

Tip cone

Troubleshooting problems associated with the pipette's tip cone.

Maintenance

Disassembling the single-channel pipette ≤ 1000 µL

Step-by-step guide for disassembling the single-channel pipette up to 1000 µL.

Disassembling the single-channel pipette ≥ 2 mL

Step-by-step guide for disassembling the single-channel pipette from 2 mL upwards.

Assembling the single-channel pipette ≤ 1000 µL

Step-by-step guide for assembling the single-channel pipette up to 1000 µL.

Assembling the single-channel pipette ≥ 2 mL

Step-by-step guide for assembling the single-channel pipette from 2 mL upwards.

Replacing the protection filter ≥ 2 mL

Procedure for replacing the protection filter in pipettes of 2 mL and above.

Disassembling the multi-channel pipette

Steps to disassemble the multi-channel pipette.

Removing the channel

Detailed instructions for removing a channel from a multi-channel pipette.

Assembling the multi-channel lower part

Steps for assembling the lower part of a multi-channel pipette.

Replacing the O-ring

Guide on how to replace the O-ring on the pipette.

Deactivating the spring loading of the tip cone

Instructions on how to turn off the tip cone spring loading feature.

Deactivating the spring load - single-channel pipettes ≤ 1000 µL

Specific steps to deactivate spring load for single-channel pipettes ≤ 1000 µL.

Deactivating the spring load - multi-channel pipettes

Specific steps to deactivate spring load for multi-channel pipettes.

Adjusting the pipette

Information on performing pipette adjustments (user and factory).

Cleaning and disinfecting the pipette

Comprehensive guide for cleaning and disinfecting the entire pipette.

Cleaning and disinfecting the lower part

Specific instructions for cleaning and disinfecting the pipette's lower section.

Sterilizing the pipette with UV light

Method for sterilizing the pipette using UV light irradiation.

Autoclaving the pipette

Procedure and precautions for autoclaving the pipette.

Autoclaving

Detailed steps and prerequisites for autoclaving the pipette.

Decontamination before shipment

Instructions for decontaminating the device prior to shipping or storage.

Technical data

Increments of the single-channel and multi-channel pipettes

Table detailing volume increments for various pipette models.

Ambient conditions

Specifies recommended temperature and humidity for storage and operation.

Measurement deviations according to Eppendorf AG

Single-channel pipette with fixed volume

Presents measurement deviation data for fixed-volume single-channel pipettes.

Single-channel pipette with variable volume

Presents measurement deviation data for variable-volume single-channel pipettes.

Multi-channel pipette

Presents measurement deviation data for multi-channel pipettes.

Test conditions

Describes the conditions and methods used for performance testing and analysis.

Ordering information

Single-channel pipettes, variable volume

Lists order numbers for variable volume single-channel pipettes by model.

Single-channel pipettes, fixed volume

Lists order numbers for fixed volume single-channel pipettes by model.

Multi-channel pipettes

Lists order numbers for multi-channel pipettes by model.

Spare parts, accessories and pipette tips

Information on how to order replacement parts and accessories.

Eppendorf Reference 2 Specifications

General IconGeneral
Ergonomic DesignYes
Volume Range0.1 µL to 10 mL
Volume Increments0.1 µL
Materialstainless steel
AutoclavableYes, fully autoclavable
DimensionsVaries by model
Power SupplyNot applicable (manual pipette)
Precision≤ 0.2% (10 µL - 10, 000 µL), ≤ 0.5% (0.1 µL - 10 µL)
AdjustmentEasy Calibration technology

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