14
Operation
Eppendorf Reference
®
2
English (EN)
4.3 Optimum immersion depths
4.4 Forward pipetting
4.4.1 Aspirating liquid
Prerequisites
• The pipette tip has been attached.
1. Press down the control button to the first stop.
2. Immerse the pipette tip vertically in the liquid.
3. Maintain the immersion depth and let the control button slide back slowly.
Liquid is aspirated into the pipette tip.
4. Wait approx. 3 seconds.
5. Remove the pipette tip from the liquid.
4.4.2 Dispensing liquid
1. Place the pipette tip on the tube inner wall at a steep angle.
2. Slowly press down the control button to the first stop.
The liquid is dispensed.
3. Wait until the flow of liquid stops.
4. Press down the control button to the second stop.
The pipette tip is completely emptied.
5. Hold down the control button and wipe the pipette tip on the tube inner wall.
4.4.3 Ejecting the pipette tip
Press the control button down all the way.
The pipette tip is ejected.
4.5 Reverse pipetting
An additional volume (blow out) is aspirated when performing reverse pipetting. This can
improve dispensing results for viscous or foaming liquids. Volume limits may occur when
using filter tips.
Volume Immersion depth
0.1 µL – 1 µL 1 mm
1 µL – 100 µL 2 – 3 mm
100 µL – 1000 µL 2 – 4 mm
1 mL – 10 mL 3 – 5 mm
To ensure maximum precision and accuracy, we recommend initially wetting each
new tip by aspirating and dispensing the liquid one to three times.
If necessary, wipe the pipette tip against the tube inner wall.
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