Eppendorf Mastercycler Gradient - Temperature Increment and Time Increment; Ramp and Ramp Increment Settings

27
7 Programming
+ 0.0 ° Temperature increment ( ± ° C): For each cycle, the temperature is increased or decreased by the
value entered here.
Please note that the temperature cannot exceed 99 ° C. For example, with a temperature
increment of +0.1 ° C and 25 cycles, the starting temperature cannot be greater than 96.5 ° C.
Entry for temperature increase "+"
Entry for temperature decrease " – "
Permitted values ± 0.0 to ± 10.0 ° C
Entry increments 0.1 ° C
+ 0:00 Time increment ( ± s): For each cycle, the time is extended or reduced by the value entered here.
Entry for time extension "+"
Entry for time reduction " – "
Permitted values 0:01 to 1:00
Entry increments 1 s
R = 3 ° /s Ramp ( ° C/s): The ramp states the speed of the heating or cooling curve of a cycler. The higher
the value entered, the more rapidly the heating or cooling takes place. Most reactions require
rapid heating or cooling rates, i.e. a high value. For processes in which heating or cooling should
take place slowly, different values may be entered for the ramp.
Permitted values 0.3 to 3 ° C/s
Entry increments 0.1 ° C/s
+ 0.0 ° /s Ramp increment ( ± s): For each cycle, the ramp is raised or lowered by the value entered here.
Please note that the ramp cannot exceed 3 ° C/s, i.e. with an initial ramp entry of 0.3 and a ramp
increment of 0.1, a maximum of 27 cycles is possible.
Permitted values
± 0.1 to ± 1.0 ° C/s
Entry increments 0.1
° C/s
Possible with Mastercycler
®
gradient only.
G = 0.0 Gradient: When an entry is made here, each column of the block is set to a different temperature.
The nominal temperature set at "T" is in the center of the block. The lowest temperature is on the
left-hand side and increases gradually up to the highest temperature on the right-hand side. This
means that a temperature difference of up to 20 ° C across the block can be generated (see
Sec. 5.4.3). The temperature distribution can be viewed in the OPTIONS/Gradient program. With
0.2 ml test tubes, a maximum of 12 different temperatures may be set whereas only 11 can be
set when 0.5 ml test tubes are used.
Please note that the temperature cannot exceed 99 ° C, i.e. if a gradient of +10 ° C is built up, the
nominal temperature may not be greater than 89 ° C.
Permitted values
± 10 ° C
Entry increments 0.1 ° C
The gradient has no effect when the
"in situ" type is used.