Eppendorf Mastercycler Gradient - Mastercycler Short Instructions; Switching on the Device; Modifying the Standard Example Program

41
8 Short instructions
It is essential to read the operating manual completely before working with the short instructions.
Switching on the device
Programming
– Select the FILES menu using the keys and press .
Changing the "Standard" example program
– Select Standard using the key and press .
– Select the program commands using the key, enter the settings or the texts and confirm by pressing .
– Using the , , , keys, move the cursor to or between the entry positions.
Program commands
T Temperatures, times, increments, ramp, gradient
HOLD To maintain temperature entered until program is continued with Enter (4–99 ° C)
PAUSE To interrupt program (e.g. for addition of reagent) until program is continued with Enter (0–11 characters)
GOTO/REP To repeat program parts and number of cycles (1–40 lines, 1–99 repeats)
SOUND Acoustic signal (1–10 tones)
LINK To branch to other programs (1–5 programs)
– Confirm entries by pressing .
– Enter the options for the temperature command using the key. The cursor must be positioned on the program line
number.
– Complete programming by pressing .
– Save the program.
CNTRL To define temperature regulation
BLOCK For the thermoblock
TUBE For different tubes
Lid To determine the lid temperature
NOWAIT To start a program immediately
WAIT Program waits for lid temperature
AUTO Lid switches off at < 22 ° C
and time > 5 min.
FIX Lid switches off at end of program
T Temperature (0–99 ° ),
Time (0:00:01–9.59:59)
Temperature increment ( ± 0.0 – ± 10.0 ° ),
Time increment (0:01–1:00),
R Ramp (0.3–3 ° C/s),
Ramp increment ( ± 0.1 – ± 1.0 ° /s)
G Gradient ( ± 10 ° C)
(for Mastercycler
®
gradient only)
Menu name
To start any program
Programming
System settings
To preheat the lid
To temperature-control block and heated lid
Program in processing level
Nominal/actual block temperature
Lid temperature
(only when lid is switched on)
Main-Menu
Start
FILES . .
OPTIONS . .
Lid
Incubate
STANDARD
– –/25.0° Lid:105° 10:22:06
/
To edit the program in the processing level
To load and process the example program
To delete any program
To load and edit any program
To create a new program
FILES . .
Edit
Load
Standard
New
Delete
STANDARD
– –/22.0° 10:22:06
FILES . . Edit STANDARD
Edit CNTRL BLOCK
Load LID=105°
Standard NOWAIT AUTO
New 1 T=94.0° 0:02:00
Delete 2 T=94.0° 0:00:15
STANDARD 3 T=44.0° 0:00:15
– –/22.0° 10:22:06
FILES . . STANDARD
Edit 3 T=44.0° 0:00:15
Load 4 T=72.0° 0:00:30
Standard +0.0° +0:01
New R= 3.0°/s +0.0°/s
Delete G=0.0°
STANDARD 5 GOTO 2 REP 24
– –/22.0° 10:22:06
8 Short instructions
Time