Eppendorf Mastercycler Gradient - Program Saving and Line Management; System Settings and Program Execution

42
8 Short instructions
Adding a program line
– Using the keys, move the cursor to the front of the line.
– Press the key; a new line is then added above the cursor.
Deleting a program line
– Using the keys, move the cursor onto the program line number.
– Press the key; the line is then deleted and the cursor moves to the next line.
System settings
To temperature-control lid and block Incubate menu Block temperature 37 ° C, Heater is ON
To preheat the lid Lid menu LidTemp 105 ° C, Heater is ON
To print programming OPTION menu Printout Editor: YES
To print program sequence OPTION menu PrintProtocol: ON
Continuing an interrupted program
– Press the key.
– Using the key, select "Resume".
– Confirm by pressing .
Saving a program
– Exit the program using the key.
– Answer the question "Save: YES"
by pressing .
– Define the program name using the key.
– Confirm the name by pressing .
Starting a program
– Switch on the device. If necessary,
preheat the lid and the block.
– Select the "Start" menu using the
keys and press .
– Select the program name using the
keys and press .
– With CNTRL TUBE, select the tube type
using the key. Enter the filling volume.
0.2 ml/plate: 5 to 50 µ l
0.5 ml/thin: 5 to 100 µ l
– Confirm by pressing .
The program starts immediately.
– To see the program run time, press the key.
Interrupting a program
– Press the key.
– Using the key, select the "Pause" program.
– Confirm by pressing .
Stopping a program
– Press the key.
– Confirm "Stop" by pressing .
– Exit the program by pressing .
FILES . . Edit STANDARD
Edit STANDARD not saved
Load SAVE: YES
Standard
ProgName: STANDARD
New
Delete
STANDARD
– –/22.0° 10:22:06
/
/
/
/
Main-Menu Run a Program:
Start STANDARD
FILES . . TEST1
OPTIONS . . TEST2
Lid
Incubate
STANDARD
– –/25.0° 10:22:06
Main-Menu Run a Program:
Start 1 T=95.0° 0:00:05
FILES . .
OPTIONS . .
Lid
Incubate
Running/STANDARD Cyc:4
95.0/94.5° Lid:105° 10:22:06
Main-Menu Run a Program:
Start STANDARD running . . .
FILES . .
Program: PAUSE
OPTIONS . .
Lid
Incubate
Paused/STANDARD
95.0/94.5° Lid:105° 10:22:06
Main-Menu Run a Program:
Start STANDARD running . .
FILES . .
Program: STOP
OPTIONS . .
Lid
Incubate
STANDARD
95.0/94.5° Lid:105° 10:22:06