Eppendorf Mastercycler Gradient - Sample Cooling and Subsequent PCR

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7 Programming
7.6.6 Sample cooling and subsequent PCR
If sensitive samples should remain cooled until the PCR takes place, the thermoblock must be preheated. This
can be carried out in two ways:
1. Set the "Incu" menu to 4 °C and "Heater is On".
2. Adapt an initial program and connect via a "LINK" command.
For this purpose, the temperature of the thermoblock is brought to 4 °C using the first program. After the
samples have been inserted into the cold thermoblock, the second program with the PCR protocol is
called up via a LINK command.
Programming should take place as follows:
Program 1 e.g. COOL
CNTRL TUBE The entries in the first program are also valid for the programs which are added
Lid via LINK. The lid setting "Fix" must be selected.
NOWAIT FIX
1 Hold 4
° C Enter The thermoblock cools until the processing program (e.g. PCRPROT) is started
via the LINK command by pressing .
2. Link PCRPROT Link to the second program with the PCR protocol.
end
Program 2 PCRPROT
CNTRL TUBE If the program also contains the "Tube control" command, further entries for
tube type and filling volume are no longer necessary; the entries made for the
first program are valid for all further programs.