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7 Programming
7.1.9 END
END appears automatically as the last line of a program. It switches off the temperature control for the block
and for the lid.
7.2 Creating a new program
The process for entering program commands is identical for the Mastercycler
®
and the Mastercycler
®
gradient. A gradient can also be programmed with the Mastercycler
®
gradient.
Example for a program sequence:
Initial denaturation 95 °C for 2 minutes
20 cycles:
Denaturation 95 °C for 45 seconds
Annealing 61 °C for 45 seconds with a temperature increment of –1 °C
A gradient of ± 10 °C is programmed (with Mastercycler
®
gradient only)
Elongation 72 °C for 45 seconds
Final elongation 72 °C for 2 minutes
Cooling of samples 22 °C
When the gradient is set at 61 °C ± 10 °C when 12 x 0.2 ml test tubes are used, the temperature distribution in
the individual columns of the thermoclock is as follows:
When 0.5 ml test tubes are used, the 11 rows of the block have the following temperatures:
Tube row 1 2 3 4 5 6 7 8 9 10 11 12
Temperature 51.0 51.3 52.5 54.2 56.5 59.1 61.8 64.5 67.0 69.1 70.6 71.5
Tube row 1 2 3 4 5 6 7 8 9 10 11
Temperature 51.1 51.9 53.3 55.4 57.8 60.5 63.2 65.8 68.1 69.9 71.1
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