Eppendorf Mastercycler Gradient - Introduction to Mastercycler

3
The Eppendorf Mastercycler
®
and Mastercycler
®
gradient are PCR devices for all molecular biological and
biochemical laboratories in the field of basic research or routine diagnostics.
Temperature control is carried out with Peltier elements, which enable extremely rapid changes in temperature
of up to 3 ° C per second. This allows the temperature of the samples to be controlled extremely rapidly
between 4 ° C and 99 ° C.
A special "tube control" mode has been developed for temperature-controlling samples in various different
tubes with various different filling volumes.
The thermoblock has been constructed as a universal block which can accommodate microtiter plates in the
8 x 12 format, 96 Eppendorf PCR test tubes of 0.2 ml, 77 thin-walled Eppendorf PCR test tubes of 0.5 ml or
other suitable tubes – without the block having to be exchanged.
With the aid of the in situ Adapter, in situ reactions may be carried out on up to four glass slides.
To prevent the formation of condensation, the devices have a heated lid which has been perfectly tailored to
suit all tubes.
The devices are easy to operate, with user guidance available in the integrated, eight-line display.
Experiments and tests can be documented with the aid of a printer which may be connected up to the devices.
One particular advantage of the Mastercycler
®
gradient is the Gradient function, which greatly facilitates the
optimization of PCR experiments. The gradient enables the temperature across the entire block to be varied
within a range of up to 20 ° C; it can be programmed with every temperature step.
.The Mastercyclers are authorized cyclers licensed by the Perkin-Elmer Corporation.
1 Introduction