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ERBA ELite 3 User Manual

ERBA ELite 3
77 pages
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Erba Lachema s.r.o., Karásek 2219/1d, 621 00 Brno, CZ
ELite 3
Hematology Analyzer
USER MANUAL
INS00066

Table of Contents

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ERBA ELite 3 Specifications

General IconGeneral
Sample positions40
Photometric range0 - 3.0 Abs
Measurement Range0 - 3.0 Abs
BatteryNot applicable
Sample TypeSerum, Plasma, Urine
Throughput200 tests/hour
Sample Volume2 - 50 μL
Display7-inch color touchscreen
ConnectivityRS-232, USB
Operating Temperature15-30 °C

Summary

1. INTRODUCTION

1.1. Intended Use

Hematology analyzer for in vitro diagnostic use, developed for small clinics and point-of-care lab offices.

1.2. The Instrument

Automated, bench-top hematology cell counter using Coulter-method for cell counting and HGB measurement.

1.5. The measurement process

Details the stages of blood testing: aspiration, dilution, WBC measurement, HGB, RBC, PLT analysis.

1.8. Specifications

Details sample volume, chambers, reagent system, throughput, accuracy, display, and power requirements.

2. INSTALLATION

2.1. General information

Instructions for installation to ensure proper operation and service. Handle with care.

2.2. Environmental factors

Operate within 15-30°C and 45-85% humidity. Avoid direct sunlight and interfering devices.

2.3. Unpacking and installation

Carefully unpack, inspect for damage, check accessories, and acclimate to room temperature.

2.3.1. Turning the Instrument ON, MAIN Menu

Turn on via rear switch. Status LED indicates operation. Wait 5 mins for optimal temperature.

2.3.2. Turning the Instrument OFF

Use SHUTDOWN from menu to prevent pneumatic failure. Never just flip power switch.

2.3.4. Handling in Emergency

In emergencies (fire, short-circuit), cut power immediately and use fire extinguisher if needed.

3. MENU SYSTEM

4. OPERATING PRINCIPLES

4.1. Impedance Method

Counts and sizes cells by detecting changes in electrical impedance as particles pass through an aperture.

4.2. Principle of HGB Measurement

Measures HGB photometrically after lysing RBCs to release hemoglobin.

4.3. Parameters

Lists and describes 20 hematology parameters measured by the analyzer, including WBC, RBC, HGB, etc.

4.4. Absolute and Linearity Ranges of Parameters

Specifies accuracy within linearity range. Exceeding range may cause errors or require pre-dilution.

5. ROUTINE UTILIZATION and MEASUREMENT

5.1. Sample handling

Use K3-EDTA for anticoagulation. Fill tubes to 7-8mm. Invert gently 8 times, do not shake.

5.2. Sample analysis

Start measurements from MEASURE screen, enter sample info. Results are stored automatically.

5.2.4. Warning Flags for WBC/HGB

Summarizes warning flags for WBC/HGB and recommended user actions.

5.2.4. Warning Flags for RBC/PLT

Summarizes warning flags for RBC/PLT and recommended user actions.

6. DATABASE

7. MAINTENANCE

7.1. Cleaning

Initiates cleaning procedures for fluidics to reduce blank value, removing contamination from tubing, chamber, valves.

7.2. Calibration

Monitors analyzer stability and verifies performance. Recommended for installation, component replacement, or periodic checks.

7.3. Quality control

Traces analyzer operation and reliability over time. Run control sample daily.

8. Diagnostics

8.1.2. Self Test

Procedure to verify proper operation of essential components. Performed at installation, after component replacement, or long disuse.

9. Settings

11. Troubleshooting

11.1. Regular Troubleshooting Procedures

Initiate maintenance procedures like blank measurement, cleaning, priming, or draining chamber.