GE AKTAprime plus - User Manual

Questions and Answers

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Brittney KingAug 2, 2025
What to do if I see baseline drift or noisy signal in my GE Laboratory Equipment?
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  Leah ReedAug 2, 2025
  If you observe baseline drift or a noisy signal, there could be several causes. Check for air in the pump or the flow cell and inspect the flow restrictor after the flow cell. Ensure tube connections are tight, and replace clamps if needed. Verify the mixer function by observing if a stirrer bar rotates when the system is running, or by running the installation test. Clean the conductivity cell with 1 M NaOH or 20% ethanol. Also, make sure the column is properly equilibrated; if not, clean it using a method suitable for column cleaning.
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Ronald GreerAug 7, 2025
How to resolve noisy UV-signal, signal drift, or instability in GE Laboratory Equipment?
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  Robert MooreAug 7, 2025
  To resolve a noisy UV-signal, signal drift, or instability, start by cleaning the UV cell with Decon™90, Deconex™11 or an equivalent solution. If the issue persists, check the buffer's purity by testing with water. Also, ensure there is no air in the pump or UV cell by purging the pump as described in the Pump User Manual and running a system wash with buffer.
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fowlerdawnAug 12, 2025
What to do if GE AKTAprime plus shows incorrect or unstable reading?
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  Tara GomezAug 12, 2025
  If you're getting incorrect or unstable readings, first ensure the conductivity flow cell cable is properly connected. Then, verify that the pump and valves are functioning correctly. If using temperature compensation, check the temperature sensor's calibration and the temperature compensation factor. Also, ensure the column is properly equilibrated and clean it if necessary. Finally, check the mixer operation by placing a stirrer bar on top of the mixer housing to see if it rotates during Run mode, or by running the installation test.
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Andrew FoxAug 18, 2025
How to fix erratic flow or irregular pressure trace in GE AKTAprime plus Laboratory Equipment?
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  jacqueline64Aug 18, 2025
  To fix erratic flow, a noisy baseline signal, or an irregular pressure trace, check all connections for leaks and ensure sufficient eluent in the reservoirs, using degassed solutions. Purge the pump. Clean the inlet or outlet check valves. If the piston seal is leaking, replace it. Additionally, check for blockages in the flow path, flushing to clear any obstructions and replacing tubing if necessary. Inspect the inlet tubing filter for clogs from unfiltered buffers or samples.
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Maria DelgadoAug 22, 2025
How to eliminate ghost peaks in GE Laboratory Equipment?
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  Rachel LiAug 22, 2025
  To eliminate ghost peaks, start by cleaning the system to remove any dirt or residues from previous runs and ensure all air is removed from the eluents. If the issue persists, clean the column according to the column's instructions to remove any residue. Also, check the mixer function by placing a stirrer bar on top of the mixer housing to ensure it rotates in Run mode or by running the installation test.
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Brian GayAug 28, 2025
What to do if GE AKTAprime plus shows slow pH response or calibration is impossible?
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  Jacob CooperAug 28, 2025
  If you experience a slow pH response or cannot calibrate, inspect the electrode glass membrane for contamination and clean it following the instructions in the Monitor pH/C-900 User Manual. If the membrane has dried out, try restoring it by soaking it in buffer overnight.

Summary

Important User Information

Instrument Overview

Monitoring and Evaluation

Safety Instructions

General Safety Precautions

Covers general precautions for safe operation, handling, and maintenance of the instrument.

Flammable Liquids and Personal Protection

Provides safety guidelines for using flammable liquids and required personal protective equipment.

Instrument Installation and Operation Safety

Details safety measures for installing, moving, and operating the instrument, including electrical safety.

Maintenance Safety

Outlines safety during maintenance, electrical hazards, and chemical handling.

Specific Safety Warnings

Covers warnings related to NaOH, pressure testing, fuses, and UV lamps.

Labels, Emergency, and Recycling

Emergency Procedures

Declaration of Hazardous Substances (DoHS)

Installation Guide

Site Requirements and Transport

Unpacking and Connections

Operation of the ÄKTAprime plus

Operation Overview and Workflow

Starting the Instrument

Provides step-by-step instructions for powering on and initializing the instrument.

Preparations Before Starting a Run

Details buffer preparation, sample preparation, and system setup before a run.

Performing a Run and Viewing Results

Guides on selecting methods, running chromatography, and viewing results in PrimeView.

Procedures After a Run

Describes essential steps to take after completing a chromatography run, including cleaning.

Maintenance

General Maintenance Guidelines

Stresses the importance of regular maintenance for safe and efficient operation.

User Maintenance Schedule

Provides a schedule for daily, weekly, and monthly maintenance tasks.

Cleaning Procedures

Explains cleaning protocols, including Cleaning-In-Place (CIP).

Component Maintenance and Disassembly

Covers maintenance of major components and safe disassembly/assembly procedures.

Calibration

Storage

Troubleshooting Guide

UV Curve Problems

Addresses common issues related to UV signal curves and their corrective actions.

Conductivity Curve Problems

Covers problems with conductivity measurements and provides solutions.

pH Curve Problems

Details common issues with pH measurements and their resolutions.

Pressure Curve Problems

GE AKTAprime plus Specifications

General

Pressure Range	0 to 5 MPa (0 to 50 bar)
pH Range	2 to 12
Software	UNICORN
Power Requirements	100-240 V, 50/60 Hz
Type	Chromatography System
UV Wavelength Range	190 to 700 nm
Conductivity Range	0.1 to 999.9 mS/cm
Detection Method	UV, Conductivity
Sample Loop Volume	5 µl - 5 ml (standard loops)
Pump Type	Dual Piston Pump