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GE AKTA avant - User Manual

GE AKTA avant
538 pages
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ÄKTA
avant
User Manual

Table of Contents

Other manuals for GE AKTA avant

Questions and Answers

GE AKTA avant Specifications

General IconGeneral
BrandGE
ModelAKTA avant
CategoryLaboratory Equipment
LanguageEnglish

Summary

Introduction

Important User Information

Provides critical safety notices, intended use, and prerequisites for operating the ÄKTA avant system.

ÄKTA avant Overview

Gives a general overview of the ÄKTA avant instrument and its main features, including UNICORN software integration.

Associated Documentation

Lists all user documentation delivered with the ÄKTA avant system, including manuals and application notes.

The ÄKTA avant Instrument

Overview Illustrations

Illustrates the main parts and configurations of the ÄKTA avant instrument, detailing standard and optional modules.

Instrument Display

Describes the location and function of the instrument display, including states, buttons, and indicators.

Accessories

Details available accessories like column holders, tubing holders, and extension boxes for system customization.

Standard Instrument Modules

Pumps

Describes the three high-precision pumps (System pumps A/B, Sample pump) and their functions and operating limits.

Mixer

Explains the mixer's function for buffer composition, its location, and available chamber volumes.

Valves, Overview

Provides a general overview of the rotary valves used in the ÄKTA avant system and their design.

UV Monitor

Details the UV monitor module, its function, wavelength range, and calibration.

Optional Instrument Modules

System Extension Overview

Outlines the stages for extending the system with additional modules to customize functionality.

Installation of Optional Modules

Provides instructions for installing optional modules and managing Node IDs within the system.

Extra Inlet Valves

Describes how to install additional inlet valves to increase the number of available sample and buffer inlets.

Fraction Collector F9-R

Details the external fraction collector F9-R, its installation, function, and capabilities.

I;O-box

Explains the function, connectors, and signals of the I/O-box for interfacing external equipment.

Operation

Before You Prepare the System

Provides a checklist of preparations and understanding required before starting system setup.

Prepare the Flow Path

Guides on preparing inlet, outlet, and waste tubing, and selecting the appropriate mixer chamber.

Start UNICORN and Connect to the System

Provides instructions on launching UNICORN software and establishing a connection to the ÄKTA avant instrument.

Prime Inlets and Purge Pump Heads

Details procedures for priming inlets and purging pump heads for both system and sample pumps.

Sample Application

Describes different techniques for sample application, including direct injection and loop-based methods.

Create a Method and Perform a Run

Guides on creating methods, performing runs, monitoring progress, and executing after-run procedures.

Performance Tests

General Performance Test Actions

Covers common actions for starting, running, and evaluating all performance tests.

Air Sensor Tests

Details the procedure for testing the functionality of the integrated and optional air sensors.

Built-in Fraction Collector Test

Describes the test to verify the functionality of the built-in fraction collector, including required materials.

Quaternary Valve Test

Explains how to test the quaternary valve's gradient formation capability through a series of gradient steps.

Maintenance

Maintenance Manager

Explains how to use the Maintenance Manager to view system information, statistics, and notifications.

Weekly Maintenance

Provides instructions for essential weekly maintenance tasks to ensure optimal system performance.

Semiannual Maintenance

Details maintenance activities recommended every six months for optimal system longevity.

Calibration Procedures

Guides on performing calibration procedures for system components like pH, pressure, conductivity, and UV monitors.

Replacement Procedures

Provides instructions for replacing user-replaceable components such as tubing, mixer, and flow cells.

Troubleshooting

Introduction to Troubleshooting

Provides an overview of troubleshooting procedures, including generating system error reports.

Troubleshooting: General Checklist

Offers a general checklist covering system, monitor, communication, and flow path checks before in-depth troubleshooting.

Troubleshooting: Monitors

Addresses common problems and solutions related to UV, conductivity, pH monitors, and pressure monitors.

Troubleshooting: Valves

Details common issues with valves, including detection, temperature, switching, and leakage problems.

Troubleshooting: Fraction Collector

Covers common problems and solutions for both the built-in and F9-R fraction collectors.

Troubleshooting: Pumps

Addresses issues related to pumps, including leaks, low flow, erratic pressure, and temperature problems.

Reference Information

System Specifications

Lists the technical and environmental specifications for the ÄKTA avant system.

Module Specifications

Details the operating data and specifications for various system modules like pumps, valves, and monitors.

Chemical Resistance Guide

Provides information on biocompatibility and chemical resistance of materials used in the ÄKTA avant instrument.

Predefined Methods and Phases

Describes predefined purification and maintenance methods, including their principles and included phases.

System Settings

Details system-specific settings for UV, conductivity, pH, pumps, air sensors, I/O-box, and fraction collection.

Using Manual Instructions

Explains how to use manual instructions for interacting with system components like pumps, monitors, and flow paths.

Ordering Information

Tubing

Lists available tubing items and their corresponding code numbers for ordering.

Fittings and Connectors

Details the available fittings and connectors with their product codes for system assembly.

Holders

Lists various holders for modules, columns, and tubing, along with their item and code numbers.

Pump Spare Parts

Provides a list of spare parts for pumps, including seal kits, piston kits, and check valve kits.

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