8.2 Troubleshooting: General Checklist
Introduction
Check the items in the following topics before starting more in-depth troubleshooting
work.
System checks
•
Is the correct system selected in UNICORN System Control? For more details, see
Section 5.3 Start UNICORN and connect to the system, on page 162.
•
Are the fans blowing at the back and at the right side of the system?
Monitor checks
•
Is the UV monitor set to the correct wavelength? Check the wavelengths that are
used in the method. For the predefined methods the wavelengths are set in the
Method Settings phase. For more details, see UNICORN Method Manual.
•
Is the air sensor sensitivity set to normal in UNICORN System Settings to avoid un-
necessary stops due to minor air bubbles? See Air sensor sensitivity, on page 65.
Instrument communication
•
Have Node IDs been set correctly for all instrument modules? To check and change
Node IDs, see Section 9.15 Check and change the Node ID of a module, on page 515.
Flow path checks
•
Is all tubing connected correctly? See Section 9.3 Tubing and connectors, on page432
and Section 5.2 Prepare the flow path, on page 157.
•
Is there leakage at any of the connections?
•
Is any tubing folded or twisted?
•
Is the inlet tubing correctly immersed in the buffer solution, beneath the liquid surface
but not too close to the flask bottom?
ÄKTA avant User Manual 29035184 AE 361
8 Troubleshooting
8.2 Troubleshooting: General Checklist
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