GE AKTA avant - Prime Q Inlets

5.4.3 Prime Q inlets
Overview
The procedure consists of the following stages:
DescriptionStage
Prime all Q inlet tubing.1
Validate priming of Q inlet tubing.2
Purge Quaternary Valve and the system pumps if pressure signal indicates
air bubbles.
3
Validate purge of Quarternary Valve and system pumps.4
End the run.5
Prime the Q inlets
Follow the instructions to prime the Q inlets.
ActionStep
Make sure that the pieces of inlet tubing marked A1, B1 and Q1-Q4 are im-
mersed in the correct buffers. The A1 and B1 positions are used for pump
synchronization and these lines should already be primed.
1
In the Manual instructions dialog box:2
•
Select Pumps and pressures:Quaternary start concentrations.
•
Set Start concentration Q1 to 100%. Make sure that the other start
concentrations are set to 0%.
•
Select Pumps and pressures:System flow and set Flow rate to 0.01
ml/min.
178 ÄKTA avant User Manual 29035184 AE
5 Operation
5.4 Prime inlets and purge pump heads
5.4.3 Prime Q inlets