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GE AKTA ready - User Manual

GE AKTA ready
227 pages
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ÄKTA
ready
Operating Instructions
Original instructions

Table of Contents

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GE AKTA ready Specifications

General IconGeneral
TypeChromatography System
ManufacturerGE Healthcare
Flow Rate Range0.001 - 100 mL/min
pH Range2 - 12
Power Requirements100-240 V AC, 50/60 Hz
Control SoftwareUNICORN
ApplicationsProtein purification, process development
Temperature Range4 to 40 °C
Sample ApplicationManual injection

Summary

1 Introduction

1.1 Important user information

Provides essential safety instructions and definitions of warnings, cautions, and notices for safe system operation.

1.2 Regulatory information

Details manufacturing information and conformity with EU Directives for ÄKTA ready.

1.3 Associated documentation

Lists generic and third-party documentation supplied with the ÄKTA ready system.

2 Safety instructions

2.1 Safety precautions

Covers general safety precautions, personal protection, and equipment handling during installation and operation.

2.2 Labels

Describes the system label, its location, and provides an example of its contents.

2.3 Emergency procedures

Provides information on emergency shutdowns and precautions during power failures.

2.4 Recycling information

Provides information on decontamination, product disposal, hazardous substances, and electrical components.

2.5 Declaration of Hazardous Substances (DoHS)

Explains the product pollution control information and the meaning of the Environment-friendly Use Period (EFUP) symbol.

3 System description

3.1 Overview

Provides an overview of the intended use, material compliance, and key features of the ÄKTA ready system.

3.2 Layout

Illustrates the ÄKTA ready system with numbered parts and their functions, including the cabinet and column trolley.

3.3 Flow chart

Illustrates the liquid flow path through the ÄKTA ready system, indicating standard and optional components.

3.4 Equipment

Explains the function and default positions of the 19 pinch valves used for flow control.

3.5 UNICORN control system

Introduces UNICORN software for ÄKTA ready control, its knowledge prerequisites, and system network configurations.

4 Installation

4.1 Included in the delivery

Details the system cabinet, column trolley, accessory kit contents, and associated products delivered with ÄKTA ready.

4.2 Site requirements

Specifies dimensions, weight, ambient environment, and precautions for installing the ÄKTA ready system.

4.3 Transport

Provides crate dimensions for ÄKTA ready and ÄKTA ready gradient, and instructions for moving the crate using lifting equipment.

4.4 Unpacking

Provides instructions for checking equipment for damage, safety precautions, and unpacking procedures.

4.5 Positioning the system

Specifies required area, access for service, and instructions for locking the system wheels after positioning.

4.6 Connections

Details connector locations on the system cabinet and provides warnings for high voltage and power connection procedures.

5 Starting ÄKTA ready

5.1 Start and log on to UNICORN

Provides step-by-step instructions for starting UNICORN software and logging into the system.

5.2 Start the system

Explains how to start the ÄKTA ready unit, including power-on procedures and indicator lamp status.

6 Flow kit installation

6.1 ÄKTA ready flow kits

Details the three parts of ÄKTA ready flow kits: inlet manifold, pump tubing, and main part with sensors.

6.2 Unpacking the flow kit

Explains the flow kit package contents and provides steps for unpacking the flow kit in a clean environment.

6.3 Mounting the flow kit

Introduces the step-by-step procedure for installing a flow kit onto the ÄKTA ready cabinet.

7 ÄKTA ready gradient

7.1 ÄKTA ready gradient pump

Describes the ÄKTA ready gradient pump, its components, and mounting location on the ÄKTA ready.

7.2 ÄKTA ready gradient flow section

Details the two parts of the gradient flow section: pump tubing and jumper tubing, for connection to system and gradient pumps.

7.3 Calibrate and operate the ÄKTA ready gradient pump

Explains pump calibration for capacity differences and the procedure for determining compensation factors.

8 Component test

8.1 Run a component test

Explains the purpose of the component test, testing sensors, flow meter, UV/conductivity, and pressure sensors.

8.2 The test report

Describes the structure and content of the flow kit installation and component test report, including header and sections.

8.3 Troubleshooting a failed component test

Provides guidance on troubleshooting a failed component test by establishing the cause and using the installation wizard.

9 Column installation

9.1 Columns

Recommends ReadyToProcess columns for ÄKTA ready and mentions other column types may require special precautions.

9.2 Installing a column

Introduces steps for installing a column, following the wizard and column-specific instructions.

9.3 Optional column test

Explains creating efficiency test methods and performing tests under stable conditions for installation report.

9.4 Optional column rinsing

Explains column rinsing methods, procedures for removing storage solution, and selecting rinsing liquid.

9.5 Adding columns to the column list

Guides on adding new columns, like 'RTP columns on request', to the UNICORN column list for the installation wizard.

10 Creating a method

10.1 The Method Editor

Introduces the Method Editor for building and editing methods using blocks and instructions.

10.2 An example of using the Method Editor

Illustrates creating a method with blocks and instructions, focusing on basic chromatography method design.

10.3 Creating a column efficiency test

Explains creating efficiency test methods and performing tests under stable conditions for installation report.

11 Run a method

11.1 Final preparations before a run

Covers precautions for hazardous substances, corrosive materials, and temperature limits before starting a run.

11.2 Starting a run

Guides on starting a run from UNICORN, checking inlets/outlets, and fine-tuning method variables.

11.3 Viewing and printing results

Explains how to view chromatograms in the UNICORN Evaluation module and customize panel layouts.

11.4 After the run

Provides instructions for disposing of flow kit and column, including washing, draining, and remounting protective caps.

12 Maintenance

12.1 Daily maintenance

Emphasizes regular maintenance for safe operation, focusing on cleaning and pH electrode calibration.

12.2 Yearly maintenance

Outlines preventive maintenance performed annually by qualified personnel, and user-checkable tasks like air filter and sensors.

12.3 Spare parts

Lists user-replaceable parts like pressure cell holder kits, air filter nuts, and wheels, and refers to ordering information.

12.4 Storage

Provides instructions for system storage, including removing flow kits, cleaning the cabinet, and switching off power.

13 Troubleshooting

System

Covers troubleshooting for computer issues like no system found or communication failure, and power/compressed air failures.

14 Reference information

14.1 Specifications

Provides general specifications for ÄKTA ready and ÄKTA ready gradient, including dimensions, weight, and voltage.

14.2 Process wetted materials

Lists materials used in ÄKTA ready components that come into contact with process liquids.

14.3 Chemical resistance

Discusses chemical compatibility for wetted parts and lists allowed chemicals and their concentrations for ÄKTA ready.

14.4 Ordering information

Provides ordering information for ÄKTA ready flow kits, gradient flow sections, accessories, and spare parts.

14.5 PID control

Introduces PID control principles for feedback control and its application in the ÄKTA ready system.

14.6 Checklists

Presents a checklist for performed tasks, including unpacking and installation of the system unit.

14.7 Health and Safety Declaration Form

Provides a form for confirming the equipment is cleaned and the area is safe for on-site service engineers.

14.8 More information

Directs users to system documentation for spare parts, accessories, method optimization, and contact information.

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