9.3 Optional column test
Introduction
A method for efficiency testing of columns can be created in the UNICORN Method Editor
(from the provided method template), or an efficiency test can be run in connection with
installation of a column, using the installation wizard, as described in Section 9.2 Installing
a column, on page 127. Efficiency testing using the wizard places the test results in the
column installation report.
Column efficiency testing, when included in the column installation, should be performed
under stable conditions, in room temperature, using the flow rates, volumes, and buffers
prescribed. Test results are presented in the installation report.
ReadyToProcess columns are already qualified by efficiency testing and deliv-
ered with individual certificates. Customized methods for efficiency testing of
other columns can be created using the supplied wizard; see Section 10.3
Creating a column efficiency test, on page 168.
Note:
Preparations
When selecting the Column Installation alternative in the column installation wizard,
the user is given the choice of also selecting the Include Efficiency Test of Column
checkbox (see Section 9.2 Installing a column, on page 127). By doing so, additional items
concerning preparation and connection of wash and test liquids will be included in the
wizard’s checklists.
The liquids required for the test are:
•
Column wash liquid — A tank with purified water connected to inlet 6. The volume
required is shown in the wizard. The volume depends on the size of the column.
•
Tracer solution — A tank with 5 l solution of 2% acetone in column wash liquid, con-
nected to inlet 5.
If test results are to be compared, use the same solvent for wash and test liq-
uids. The wizard recommends using either water, 20% ethanol or 2% benzyl
alcohol. The choice of solvent will affect test results, ethanol will produce
lower values compared to water.
Note:
ÄKTA ready Operating Instructions 28960345 AD 135
9 Column installation
9.3 Optional column test
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