10.2.9 Block 8: Elution
Similar to sample load and washing in previous blocks, elution is controlled by UV re-
sponse. You thus need to define two watch blocks with UV-based criteria for starting
and stopping the elution process. These blocks, in turn, refer to two external blocks,
Fraction and Fraction_Stop.
The Fraction block
Start by defining the external block Fraction:
ActionStep
Create a block (in group Other), name it Fraction, and set its Base to Vol-
ume (it does not matter where the block is created, it will be moved in a later
later step).
1
Insert an OutletFractions instruction into the block, with the number of
fractions set to 1 (in this example only one fraction is retrieved).
2
Set the volume to, for instance, 8 liters, and set the fraction outlet to Outlet2.3
Once defining the Fraction block is complete, right-click the block and click Delete. A di-
alog box is displayed, in which you can choose to Delete or Move the block; click Move.
The block will be stored as Unused in the location you select, and will be available for
insertion in any method.
The Fraction_Stop block
The procedure for creating the Fraction_Stop block is very similar to that of the Fraction
block. There are two main differences: The Fraction_Stop block has SameAsMain (i.e.,
column volumes) as Base, not Volume, and the number of fractions in the OutletFractions
instruction is set to 0 (zero). Setting the number of fractions to 0 means that fractionation
will stop.
Store the Fraction_Stop block as Unused for the time being.
164 ÄKTA ready Operating Instructions 28960345 AD
10 Creating a method
10.2 An example of using the Method Editor
10.2.9 Block 8: Elution
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