10.2.10 Block 9: Regeneration
The last step for a complete chromatog-
raphy cycle is to regenerate the column.
This is done by defining a block with in-
structions for selecting inlet and outlet,
and for setting the air trap and column
inline.
The finishing criterion is based on SameAsMain (i.e., column volumes) and is defined
in a nested block. The procedure is very similar to that of defining a block for washing
unbound sample (see Section 10.2.8 Block 7: Washing unbound sample, on page163).
When, as in this case, a similar block already exists, time can be saved by copying
the existing block, renaming it, and changing the parameter values as required.
166 ÄKTA ready Operating Instructions 28960345 AD
10 Creating a method
10.2 An example of using the Method Editor
10.2.10 Block 9: Regeneration