9.4 Optional column rinsing
Column rinsing
A method for column rinsing may be custom designed by the user, or the predefined
method for column rinsing can be used. The predefined method is included as an optional
step in the column installation wizard.
Column rinsing is performed with 6 column volumes to remove all remaining storage
solution or tracer solution from the column.
Preparations
If column rinsing was selected in the installation wizard (i.e., Include Rinsing of Column
was checked) the wizard will include a field for selecting the inlet used for the rinsing
liquid.
The rinsing liquid may consist of, for instance, purified water or a low salt buffer (see
column documentation for details). The volume required depends on the column used.
Note that the liquid used for rinsing may be the same as that used as wash liquid in a
previous efficiency test (see Section 9.3 Optional column test, on page 135). In such case,
the same inlet is selected.
Run the rinsing procedure
Column rinsing takes approximately 80 minutes. No actions from the user are required
during this time. It is possible to enter notes during a run.
138 ÄKTA ready Operating Instructions 28960345 AD
9 Column installation
9.4 Optional column rinsing
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