5.7.2 Sample application using a Superloop
Introduction
A Superloop allows injection of large sample volumes onto the column. A Superloop can
also be used for multiple injections, for example in a scouting experiment when the same
application conditions are required. Superloop models are available in 10 ml, 50 ml and
150 ml sizes.
A superloop can be connected to either the Injection valve or the Loop valve. When using
the Loop valve, up to five loops can be connected simultaneously.
After loading a Superloop, always plug the Syr port on the Injection valve with
a Stop plug. With a Superloop connected to the valve, an over-pressure may
be created during injection.
Note:
Prepare the Superloop
To avoid injecting air into the system flow path, the Superloop should be prefilled with
buffer manually, before fitting the Superloop to the system.
Read the instruction for the Superloop to be used.
Note:
Connect the Superloop
The following steps describe how to connect the Superloop to the Injection valve or to
the Loop valve.
ActionStep
Attach the Superloop to the instrument using a Column holder.1
Connect a piece of tubing from the top of the Superloop to:2
•
port LoopE on the Injection valve
or
•
an E port, e.g., 1E, on the Loop valve
194 ÄKTA avant User Manual 29035184 AE
5 Operation
5.7 Sample application
5.7.2 Sample application using a Superloop
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