6.5 Quaternary valve test
Method description
The Q valve test checks the functionality of the Quaternary valve. Correct gradient for-
mation is tested by producing a series of gradient steps. The method run takes approxi-
mately 30 minutes for ÄKTA avant 25 and 70 minutes for ÄKTA avant 150.
Required material
The following material is required:
•
1% acetone in distilled water
•
Distilled water
•
Capillary loop Ref 1
•
Syringe, 25 to 30 ml
•
For ÄKTA avant 25: Mixer, 1.4 ml
•
For ÄKTA avant 150: Mixer, 5 ml
Prepare the Q valve test
Follow the instructions to prepare the system before method start.
ActionStep
Immerse the pieces of inlet tubing marked Q1 and Q3 in 1% acetone in dis-
tilled water.
1
Immerse the pieces of inlet tubing marked Q2 and Q4 in distilled water.2
Connect the capillary loop marked Ref 1 between Column valve ports 1A
and 1B to generate a back pressure.
3
For ÄKTA avant 25: Make sure that the Mixer with a chamber volume of 1.4
ml is installed.
4
For ÄKTA avant 150: Make sure that the Mixer with a chamber volume of 5
ml is installed.
For information on how to change Mixer, see Section 7.8.2 Replace the Mixer,
on page 338.
Prime all Q inlets and purge the System pumps. See Section 5.4.3 Prime Q
inlets, on page 178.
5
ÄKTA avant User Manual 29035184 AE 253
6 Performance tests
6.5 Quaternary valve test
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