ActionStep
Connect a 25 to 30 ml syringe to one of the purge valves of either of the
system pumps. Make sure that the syringe fits tightly into the purge connec-
tor.
3
Open the purge valve by turning it counterclockwise about 3 quarters of a
turn. Draw 10 ml of liquid into the syringe. Check that the Q1 inlet is filled
with liquid.
4
Close the purge valve by turning it clockwise. Disconnect the syringe and
discard its contents.
5
Repeat steps 2 to 5 for Q2, Q3 and Q4 respectively by setting the respective
Quaternary start concentration to 100%.
Tip:
The inlet tubing that is immersed in distilled water should be the last piece of
inlet tubing to be primed.
6
Tip:
If you will perform a BufferPro run, end with either Q1 or Q2.
Check that there is no air left in the pump by following the instructions in
Validate prime or purge of System Pump A or B or Sample Pump, on page172.
If air bubbles are indicated, follow the instructions in Purge Quaternary Valve
and the system pumps, on page 180.
7
ÄKTA avant User Manual 29035184 AE 179
5 Operation
5.4 Prime inlets and purge pump heads
5.4.3 Prime Q inlets
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