Purge Quaternary Valve and the
system pumps
If the priming was done thoroughly and the final buffer was drawn all the way into the
syringe and the validation of the priming showed that there was no air left in the pump
it is not necessary to purge Quaternary Valve and the system pumps.
However, if the pressure signal indicated air bubbles left in the valve or the pump, follow
these instructions to purge Quaternary Valve, System Pump A and System Pump B. Note
that both pump heads of each system pump have to be purged.
ActionStep
In the Manual instructions dialog box:1
•
Select Pumps and pressures:Pump wash, and click All in the BufferPro
/ Q inlets list.
Result: A simultaneous pump wash of all the Q inlets is started. This will re-
move air from Quaternary Valve.
Wait until the pump wash is completed.2
•
Select Pumps and pressures:Quaternary start concentrations.
•
Set Start concentration Q1 to 100%. Make sure that the other start
concentrations are set to 0%.
Select Pumps and pressures:System flow and set Flow rate to 0.01 ml/min.3
180 ÄKTA avant User Manual 29035184 AE
5 Operation
5.4 Prime inlets and purge pump heads
5.4.3 Prime Q inlets
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