6 Troubleshooting
6.1 UV curve problems
Corrective actionPossible causeError symptom
Clean the system. Make sure air is
removed.
Dirt or residues in the
flow path from previ-
ous runs. Air in the
eluents.
Ghost peak
Clean the column according to the
column instructions.
Residue in the column
from previous runs
Check the mixer function by plac-
ing a stirrer bar on top of the mixer
housing. The stirrer bar should ro-
tate when the system is in Run
mode. The mixer function can also
be checked by running the installa-
tion test.
Incorrect mixer func-
tion
Clean the UV cell by flushing
Decon™ 90, Deconex™ 11 or
equivalent.
Dirty UV cellNoisy UV-signal, sig-
nal drift or instability
Check if the signal is still noisy with
water.
Impure buffer
Purge the pump according to
Pump User Manual. Run a system
wash with buffer.
Air in the pump or in
the UV cell
50 ÄKTAprime plus Operating Instructions 28-9597-89 AB
6 Troubleshooting
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