• Press Start to activate the 2 minutes timer or wait for 2 minutes (without shaking the vials) to allow
the reaction to complete.
• Remove the caps from two Reagent Vials (red label).
• Add exactly 2.0 mL of digested sample (sample vial) to one Reagent Vial (sample vial), and 2.0 mL
of digested blank (blank vial) to the other Reagent Vial (blank vial), while keeping the vials at a
45-degree angle.
• Replace the cap tightly and invert the vials 10 times.
Warning: The vials will become hot during mixing, be careful when handling them.
Note: The method is technique sensitive: to obtain most reproducible results
it is strongly recommended to follow carefully the “invert” procedure
described on page 10.
• Place the blank vial into the holder and push it completely down.
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