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Hitachi ZA3000 Series User Manual

Hitachi ZA3000 Series
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INSTRUCTION MANUAL
FOR
MODEL ZA3000 SERIES POLARIZED ZEEMAN
ATOMIC ABSORPTION SPECTROPHOTOMETER
(OPERATION MANUAL : FLAME ANALYSIS)
Hitachi High-Technologies Corporation
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan
2nd Edition, December 2012
Copyright C Hitachi High-Technologies Corporation 2012. 1st Edition, 2012
All rights reserved. Printed in Japan. Part No. 7J1-9007-1 HF-TF (HK-HMS)

Table of Contents

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Hitachi ZA3000 Series Specifications

General IconGeneral
ModelZA3000 Series
Resolution0.1 nm
CategorySpectrophotometer
Optical SystemDouble beam
MonochromatorCzerny-Turner mount
Spectral Bandwidth0.1, 0.2, 0.5, 1, 2 nm
Bandwidth0.1, 0.2, 0.5, 1.0, 2.0 nm (selectable)
Repeatability≤0.05 nm
Light SourceDeuterium lamp
DetectorPhotomultiplier tube
Detector TypePhotomultiplier tube
Data Interval0.1 nm
Weight45 kg
Power SupplyAC 100 to 240 V, 50/60 Hz
Operating Temperature15 to 35 °C
Operating Humidity20-80%
InterfaceUSB

Summary

Preface

About This Manual

Overview of the manual's structure and content.

SAFETY SUMMARY

Definition of Alert Symbol and Signal Word

Explains symbols and signal words used for warnings.

1. START UP

1.1 Check of Instrument

Verification of instrument connections and readiness.

1.2 Mounting of Hollow Cathode Lamps

Procedure for installing lamps into the turret.

1.3 Supply of Gases

Instructions for connecting and setting gas supply.

1.4 Power-on

Steps to turn on the instrument and PC.

1.5 Power-on of Exhaust Duct

Procedure for activating the ventilation system.

1.6 Basic Operation Flow

Diagram illustrating the sequence of instrument operations.

2. STARTUP OF SOFTWARE

2.1 Startup of Atomic Absorption Spectrophotometer Program

Procedure for launching the main software application.

2.2 Setting the Instrument Online

Configuring software to communicate with the instrument.

2.3 Fundamental Operation of Software Windows

Basic interaction with software windows and elements.

2. STARTUP OF SOFTWARE

2.3 Fundamental Operation of Software Windows

Basic interaction with software windows and elements.

2.3.1 Clicking and Double-clicking

Mouse interaction techniques for software operation.

2.3.2 Names and Functions of Window Elements

Description of UI components like menu, toolbar, toolbox.

2.3.3 How to Input Measurement Conditions

Methods for entering analysis parameters into the software.

2.3.4 Opening Files

Procedure for loading existing method or data files.

2.3.5 Saving Files

Procedure for saving method or data files.

2.3.6 Asking for Help

Accessing software help features.

3. MEASUREMENT WITH CONDITIONS INPUT

3.1 Generation of Measurement Conditions

Creating or modifying analysis parameter sets.

3.2 Verification of Measurement Condition

Validating the configured analysis parameters.

3.3 Monitoring of Measurement Process

Observing real-time progress and data during measurement.

3.4 Wavelength Adjustment

Optimizing the instrument's wavelength setting.

3.5 Check of Optical Axis of Atomizer

Ensuring correct alignment of the atomizer components.

3.6 Supply of Cooling Water

Procedure for ensuring proper instrument cooling.

3.7 Ignition of Flame

Steps to ignite the flame for analysis.

3.8 Start of Measurement

Initiating the actual sample measurement process.

3. MEASUREMENT WITH CONDITIONS INPUT

3.8 Start of Measurement

Initiating the actual sample measurement process.

3.9 Change of Monitor Graph Display

Customizing how measurement data is visualized.

3.10 Clearing of Monitor Graph

Resetting the monitor graph display.

3.11 Changing the Display of a Profile Graph

Customizing the appearance of profile graphs.

3.12 Changing the Display of a Working Curve Graph

Customizing the appearance of working curve graphs.

3.13 Termination of Measurement

Procedures for properly ending a measurement sequence.

3.1 Generation of Measurement Conditions

3.1.1 Displaying the Method Window

Opening the primary interface for setting conditions.

3.1.2 Setting Measurement Mode

Selecting the analysis mode (e.g., Flame, Graphite Furnace).

3.1.3 Setting Analyte Element

Choosing the elements to be analyzed.

3.1.4 Instrument Setup

Configuring instrument-specific parameters.

3.1.5 Setting of Analytical Conditions

Defining specific parameters for analysis (e.g., fuel flow, burner height).

3.1.6 Setting Standards Table

Inputting concentrations and details for standard samples.

3.1.7 Setting Sample Table

Defining sample IDs, names, and other properties.

3.1.8 Setting Autosampler

Configuring autosampler parameters for automated analysis.

3.1.9 Setting QC Conditions

Establishing parameters for quality control checks.

3.1.10 Setting Report Format

Configuring the output format for measurement results.

3.1.11 Check of Analytical Conditions

Reviewing all set measurement parameters.

3. MEASUREMENT WITH CONDITIONS INPUT

3.8 Start of Measurement

Initiating the actual sample measurement process.

3.9 Change of Monitor Graph Display

Customizing how measurement data is visualized.

3.10 Clearing of Monitor Graph

Resetting the monitor graph display.

3.11 Changing the Display of a Profile Graph

Customizing the appearance of profile graphs.

3.12 Changing the Display of a Working Curve Graph

Customizing the appearance of working curve graphs.

3.13 Termination of Measurement

Procedures for properly ending a measurement sequence.

4. CHECKING THE RESULT

4.1 Data Table

Viewing and editing raw measurement data in tabular format.

4.2 Profile Graph

Visualizing atomization signal profiles for analysis.

4.3 Working Curve Graph

Displaying and analyzing calibration curves.

4.4 Saving and Printout of Result

Storing and outputting measurement data and reports.

4.5 Analysis and Recalculation of Data

Performing post-measurement data manipulation.

4.6 Use of the Data in Other Applications

Exporting data for use in external software.

4.1 Data Table

4.1.1 Error Marks

Interpreting and addressing error indications in results.

4.1.2 Correction of Operator, Analysis Name, and Comment

Editing metadata associated with measurements.

4.3 Working Curve Graph

4.3.1 Displaying Details of Working Curve

Accessing parameters and formulas of the working curve.

4.4 Saving and Printout of Result

4.4.1 Saving the Data

Procedure for saving measurement results to a file.

4.4.2 Printout of Result

Generating printed reports of measurement outcomes.

4.4.3 Printing Format of Profile

Configuring the layout for printing profile graphs.

4.5 Analysis and Recalculation of Data

4.5.1 Deleting and Restoring Data

Managing data entries by deleting or restoring.

4.5.2 Exchanging Data

Swapping sample data entries.

4.5.3 Changing Calculation Mode

Selecting different methods for data calculation.

4.5.4 Changing the Concentration Correction Factor

Adjusting factors for concentration calculations.

4.5.5 Changing Sample Name

Editing sample identification names.

4.5.6 Changing Sample Unit

Modifying units for sample data.

4.5.7 Changing Digits Below Decimal Point

Adjusting decimal precision for data.

4.5.8 Editing Reagent Name

Modifying names of used reagents.

4.5.9 Restoring the Recalculated Results

Reverting data to a previous recalculated state.

4.5.10 Detailed Display of Profile Graph

Viewing detailed profile graph information.

4.5.11 Specifying a Sample Blank

Using a blank sample for background correction in analysis.

4.6 Use of the Data in Other Applications

4.6.1 Output in Text Format or Excel Format

Exporting data to standard file formats.

4.6.2 Customize by MS Excel

Advanced data export and formatting for Excel.

4.6.3 Customizing the Template

Modifying Excel templates for custom reports.

5. USING VARIOUS FUNCTIONS

5.1 Various Measurement Methods

Descriptions of measurement techniques beyond the standard working curve.

5.2 Using Stock Standard Solutions

Utilizing stock solutions for preparing standards.

5.3 Addition of Matrix Modifier

Procedure for adding matrix modifiers to samples.

5.4 Sequential Injection of Samples

Injecting multiple samples sequentially into the cuvette.

5.5 Twin Injection

Using dual injection for improved measurement efficiency.

5.6 Cleaning Cuvette

Procedures for cleaning the graphite cuvette.

5.7 Sample Bumping Detection

Identifying and mitigating sample bumping during heating.

5.8 Correction of Working Curve Drift (Reslope function)

Adjusting working curves for sensitivity changes over time.

5.9 Concentrating Sample

Enhancing low concentration signals by repeating injection/drying.

5.10 Diluting Sample

Performing sample dilution automatically via autosampler.

5.11 Heating Injection of Sample

Using heated injection to improve sample dispersion.

5.12 Measurement under the Same Conditions (Copy Method)

Replicating previous measurement conditions for new samples.

5.13 Using an Existing Working Curve

Applying previously generated calibration curves.

5.14 Insertion and Remeasurement of Sample

Adding or re-measuring samples during a run.

5.15 Setting Automatic Start

Configuring the software to start automatically.

5.16 Power/Water Saving by Eco Mode

Activating energy-saving features.

5.17 Restoring Data Lost due to a Power Failure

Recovering measurement data after power interruption.

5.1 Various Measurement Methods

5.1.1 Absorbance Mode

Measuring absorbance without generating a working curve.

5.1.2 Standard Addition Method

Quantitative analysis using standard addition calibration.

5.1.3 Simple Standard Addition Method

A simplified standard addition technique.

5.14 Insertion and Remeasurement of Sample

5.14.1 Insertion of Samples

Adding new samples to the sequence mid-run.

5.14.2 Remeasurement

Re-running specific samples within a sequence.

6. USING THE QC FUNCTIONS

6.1 Using Correlation Coefficient of Working Curve (Check Working Curve)

Validating calibration curve quality using correlation.

6.2 Using the Working Curve Range and RSD (Check Sample)

Checking unknown sample concentration against calibration range and RSD.

6.3 Using Sensitivity Variations of Standard Solution (Check STD)

Monitoring calibration sensitivity using standard samples.

6.4 Using the QC Sample (Check QC Sample)

Analyzing quality control samples to ensure accuracy.

7. TERMINATING OR CONTINUING MEASUREMENT

7.1 Continuing Measurement with Element Change

Switching to the next element in a multi-element sequence.

7.2 Terminating Measurement

Steps to properly stop an ongoing measurement sequence.

7.3 Closing AAS program

Shutting down the AAS software application.

7.4 Closing Operations

Final steps to safely shut down the instrument.

8. INSTRUMENT DIAGNOSIS

8.1 Items to Be Prepared by the User

List of necessary materials for instrument diagnosis.

8.2 Diagnostic Items

Details of the performance parameters checked during diagnosis.

8.3 Operation

Step-by-step guide for performing instrument diagnosis.

9. TROUBLESHOOTING

Flame not ignited

Troubleshooting steps for when the flame fails to ignite.

The [Start] button is shown grayed out.

Resolving issues where the start button is inactive.

Desired sensitivity not attainable

Diagnosing and fixing low sensitivity issues.

Poor repeatability

Addressing inconsistent measurement results.

Rate of recovery improper

Troubleshooting issues with sample recovery rates.

Baseline not stable

Resolving problems with unstable baselines.

Noise is large.

Addressing excessive signal noise.

If the service life of graphite cuvette is short.

Troubleshooting premature cuvette wear.

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