Hitachi UH5300 User Manual (English)

Questions and Answers:

J
Jordan HernandezAug 6, 2025
Why is the reproducibility poor in Hitachi Measuring Instruments?
- C
  Christian CastilloAug 6, 2025
  Poor reproducibility in Hitachi Measuring Instruments can stem from several factors. Bubbles in the flow cell or the sample itself, insufficient sipped amount, contamination in the flow cell or tubing, temperature sensitivity of the sample, residual sample in the instrument, instability of the liquid in the flow cell, high sample viscosity, or an open sample compartment cover can all contribute. To address this, try washing the flow cell, removing bubbles and dust from the sample, increasing the sipping time, ensuring a constant measurement temperature, extending the delay time, cleaning the tubing, diluting the sample to reduce viscosity if needed, and ensuring the sample compartment cover is closed during measurements.
C
Christopher JenningsAug 12, 2025
What to do if the Hitachi Measuring Instruments photometer does not start?
- J
  Jamie JacksonAug 13, 2025
  If your Hitachi photometer isn't starting, even with the power switch on, there could be a few reasons. First, check if the power cord is securely plugged in. If it is, the fuse might be blown, requiring a replacement. If neither of these is the issue, try turning the power switch off and then on again. If the problem persists, it's recommended to contact Hitachi High-Technologies Corporation sales representative.
J
Julie PerryAug 18, 2025
What to do if sipping operation does not cause the peristaltic pump to work on Hitachi UH5300 Measuring Instruments?
- G
  garciaryanAug 18, 2025
  If the sipping operation isn't causing the peristaltic pump to work on your Hitachi Measuring Instruments, it could be due to incorrect installation of the Auto Sipper or a missing coupling shaft. Reinstall the Auto Sipper and attach the coupling shaft.
H
Heather BrownAug 23, 2025
How to fix if the Auto Sipper tab does not appear in the measurement condition setting of Hitachi UH5300?
- C
  Christopher HallAug 25, 2025
  If the Auto Sipper tab is missing from the measurement condition settings of your Hitachi Measuring Instruments, the connection cord between the Auto Sipper and the main unit might not be properly plugged in. Ensure the connection cord is correctly installed. If the issue persists, try turning the power off and then on again. If the tab still doesn't appear, contact Hitachi High-Technologies Corporation sales representative.
B
barbaraadamsAug 30, 2025
What to do if the sipping operation causes the peristaltic pump to rotate but the sample is not sipped in Hitachi UH5300 Measuring Instruments?
- S
  stephencameronAug 31, 2025
  If, during the sipping operation, the peristaltic pump rotates but the sample isn't being sipped in your Hitachi Measuring Instruments, several factors could be at play. Check if the pinchcock on the drain tube is closed and open it. The peristaltic tube might be deteriorated and need replacement. Ensure the peristaltic tube is correctly installed. Also, verify that the IN-joint is tightened properly. If the sample's viscosity is high, consider lengthening the sipping time or diluting the sample.

Hitachi UH5300 Specifications

General

Brand	Hitachi
Model	UH5300
Category	Measuring Instruments
Language	English

Summary

PREFACE

ABOUT THIS INSTRUCTION MANUAL

Overview of the content and purpose of this instruction manual.

IMPORTANT

Product Warranty

Details of the product warranty, including scope, period, and limitations.

Limitations and Exclusions on Warranty

Cases excluded from free-of-charge repair coverage during the warranty period.

Disclaimer of Warranty

Manufacturer's disclaimers regarding warranties and liabilities.

Service Life of This Instrument

Estimated useful service life and safety check requirements after its expiration.

Installation/Relocation and After-sale Technical Service

Installation and Relocation

Procedures and considerations for installing and relocating the instrument.

After-sales Service

Information on obtaining service and maintenance after the warranty period.

Technical Seminars and Training for Customers

Caution on Disposal of Instrument

Other Precautions

Handling of Chemicals and Samples

Guidelines for safe handling, storage, and disposal of chemicals and samples.

SAFETY SUMMARY

General Safety Guidelines

General safety instructions to be read before using the Auto Sipper.

DANGER Indications

Explanation of DANGER signal words and their applicability.

WARNING Indications

Explanation of WARNING signal words and their applicability.

CAUTION Indications

Explanation of CAUTION signal words and their applicability.

Common Safety Precautions

General safety precautions to be observed before, during, and after instrument use.

Common Safety Precautions (Continued)

Further common safety precautions regarding hazard awareness and protective equipment.

Common Safety Precautions (Continued)

Precautions on maintenance, relocation, warning labels, and life-limited parts.

Safety Instructions in This Manual

Summary of safety instructions within the manual and their relevant sections.

Injury due to Entanglement

Warning about potential entanglement hazards with the peristaltic pump.

Fatigue due to Long Hours of Operation

Advice on avoiding fatigue during prolonged instrument operation.

NOTICE

Disposal of Waste Solution

Guidelines for proper disposal of waste solutions and environmental regulations.

Accuracy and Precision of Measured Values

Recommendations for periodic inspection to maintain accuracy and precision.

Electricity

Requirements and precautions for power supply and electrical grounding.

NOTICE (Continued)

Power Interruption

Recommendations for preventing data loss due to power failure or voltage drops.

1 INSTALLATION

1.1 Outline

Overview of the Auto Sipper's function and integration with the spectrophotometer.

1.2 Checking before Mounting Auto Sipper

Pre-installation checks including environmental conditions and parts verification.

1.2.1 Installation Conditions

Environmental conditions required for instrument installation.

1.2.2 Checking against Packing List

Procedure to verify all delivered parts are present and undamaged.

1.3 Mounting Auto Sipper in Sample Compartment

Detailed procedure for installing the Auto Sipper into the spectrophotometer's sample compartment.

1.3.1 Dismounting 6 Cell Turret

Steps to remove the 6 cell turret from the sample compartment.

1.3.2 Dismounting Cell Holder for Reference

Procedure to remove the cell holder for reference from the sample compartment.

1.3.3 Removing Sample Compartment Front Cover

Steps to remove the front cover of the sample compartment.

1.3.4 Attaching Front Cover for Accessory Device

Procedure to attach the front panel for accessory devices.

1.3.5 Attaching Coupling Shaft

Steps to attach the coupling shaft to the drive motor shaft.

1.3.6 Mounting Auto Sipper

Detailed steps for mounting the Auto Sipper main unit.

1.4 Reverse Process to Change Auto Sipper to 6 Cell Turret

Steps to remove the Auto Sipper and reinstall the 6 cell turret.

1.4.1 Unplugging power cord

Procedure to safely unplug the instrument's power cord.

1.4.2 Dismounting Auto Sipper

Steps to dismount the Auto Sipper from the main unit.

1.4.3 Detaching Coupling Shaft

Procedure to detach the coupling shaft.

1.4.4 Detaching Front Panel

Steps to remove the front panel.

1.4.5 Attaching Sample Compartment Front Cover

Procedure to reattach the sample compartment front cover.

1.4.6 Mounting Cell Holder for Reference

Steps to mount the cell holder for reference.

1.4.7 Mounting 6 Cell Turret

Procedure to mount the 6 cell turret.

2 FUNCTIONS

2.1 Application

Describes the application of the Auto Sipper with the Model UH5300 Spectrophotometer.

2.2 Mechanism

Details the operational mechanism and components of the Auto Sipper system.

2.2.1 Operational Mechanism

Explanation of the Auto Sipper system's operational mechanism and components.

2.2.2 Details of Measurement Section and Liquid Delivery Mechanism

Detailed description of the measurement section and liquid delivery components.

2.2.3 Setting Peristaltic Tube

Step-by-step instructions for setting the peristaltic tube.

2.2.4 Releasing Peristaltic Tube

Procedure to detach the peristaltic tube from the roller.

2.3 Details of Operation Keys, etc.

Explains the functions of various operation keys and lamps.

2.3.1 Sipping Lever and SIPPING Lamp

Description of the sipping lever and SIPPING lamp functions.

2.3.2 RETURN Key

Explanation of the RETURN key function for sample return.

2.3.3 WASH Key

Details of the WASH key function for cleaning and pre-conditioning.

3 OPERATION

3.1 Preparation

Steps to prepare the instrument and environment before operation.

3.2 Setting Measurement Conditions

Guide to setting measurement parameters like Sipping Time, Delay Time, and Air Purge.

3.2.1 Selecting Measurement Item

Guide to selecting the appropriate measurement item from the menu.

3.2.2 Setting Auto Sipper

Configuration settings for the Auto Sipper, including Sipping Time, Delay Time, and Air Purge.

3.3 Measurement

Procedures for various measurement types: Concentration, Wavelength Scan, Time Scan, Monitor.

3.3.1 Precautions for Measurement

Important precautions to ensure accurate measurements and prevent carry-over.

3.3.2 Concentration Measurement

Procedure for quantitative calculation to create calibration curves.

3.3.3 Wavelength Scan

General procedure for performing a wavelength scan measurement.

3.3.4 Time Scan

General procedure for performing a time scan measurement.

3.3.5 Monitor Measurement

General procedure for performing a monitor measurement.

3.4 Checking of Sipping Amount

Procedure and criteria for checking the sipping amount and peristaltic tube condition.

3.4.1 Specification to be Checked

Specification details for checking the sipping amount.

3.4.2 Signs for Replacement of Peristaltic Tube

Indicators for replacing the peristaltic tube due to wear or damage.

3.4.3 Checking Conditions

Conditions required for checking the sipping amount.

3.4.4 Checking Procedure

Step-by-step procedure for checking the sipping amount.

3.5 After Measurement

Steps to follow after completing a measurement, including cleaning the flow cell.

3.5.1 Cleaning Flow Cell

Procedure for cleaning the flow cell after measurement.

3.6 Specifications

Lists the technical specifications of the Auto Sipper.

4 MAINTENANCE

4.1 Periodic Maintenance

Procedures for routine maintenance to ensure instrument performance and longevity.

4.1.1 Precautions for Measurement

Precautions to ensure measurement reproducibility by cleaning the flow cell.

4.2 Maintenance of Peristaltic Tube

Instructions for maintaining, replacing, and checking the peristaltic tube.

4.2.1 About Peristaltic Tube

Information regarding the Tygon peristaltic tube and its replacement criteria.

4.2.2 Peristaltic Tube Replacement Procedure

Detailed steps for replacing the peristaltic tube.

4.2.3 Checking Chemical Resistance of Peristaltic Tube

Procedure to check chemical resistance of the peristaltic tube against samples.

4.3 Precautions on Using Maintenance Menu

Important precautions when operating the maintenance menu features.

4.4 Replacement Parts

Information on consumable and timely replacement parts.

4.4.1 Consumables

Information on consumable items and ordering procedures.

4.4.2 Timely Replacement Item

Details of items requiring timely replacement due to wear or damage.

4.5 Troubleshooting

Guide to diagnosing and resolving common instrument issues and symptoms.

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522 pages

Hitachi UH5300 User Manual

Table of Contents

Questions and Answers:

Hitachi UH5300 Specifications

Summary

PREFACE

ABOUT THIS INSTRUCTION MANUAL

IMPORTANT

Product Warranty

Limitations and Exclusions on Warranty

Disclaimer of Warranty

Service Life of This Instrument

Installation/Relocation and After-sale Technical Service

Installation and Relocation

After-sales Service

Technical Seminars and Training for Customers

Caution on Disposal of Instrument

Other Precautions

Handling of Chemicals and Samples

SAFETY SUMMARY

General Safety Guidelines

DANGER Indications

WARNING Indications

CAUTION Indications

Common Safety Precautions

Common Safety Precautions

Common Safety Precautions (Continued)

Common Safety Precautions (Continued)

Safety Instructions in This Manual

Injury due to Entanglement

Fatigue due to Long Hours of Operation

NOTICE

Disposal of Waste Solution

Accuracy and Precision of Measured Values

Electricity

NOTICE (Continued)

Power Interruption

1 INSTALLATION

1.1 Outline

1.2 Checking before Mounting Auto Sipper

1.2.1 Installation Conditions

1.2.2 Checking against Packing List

1.3 Mounting Auto Sipper in Sample Compartment

1.3.1 Dismounting 6 Cell Turret

1.3.2 Dismounting Cell Holder for Reference

1.3.3 Removing Sample Compartment Front Cover

1.3.4 Attaching Front Cover for Accessory Device

1.3.5 Attaching Coupling Shaft

1.3.6 Mounting Auto Sipper

1.4 Reverse Process to Change Auto Sipper to 6 Cell Turret

1.4.1 Unplugging power cord

1.4.2 Dismounting Auto Sipper

1.4.3 Detaching Coupling Shaft

1.4.4 Detaching Front Panel

1.4.5 Attaching Sample Compartment Front Cover

1.4.6 Mounting Cell Holder for Reference

1.4.7 Mounting 6 Cell Turret

2 FUNCTIONS

2.1 Application

2.2 Mechanism

2.2.1 Operational Mechanism

2.2.2 Details of Measurement Section and Liquid Delivery Mechanism

2.2.3 Setting Peristaltic Tube

2.2.4 Releasing Peristaltic Tube

2.3 Details of Operation Keys, etc.

2.3.1 Sipping Lever and SIPPING Lamp

2.3.2 RETURN Key

2.3.3 WASH Key

3 OPERATION

3.1 Preparation

3.2 Setting Measurement Conditions

3.2.1 Selecting Measurement Item

3.2.2 Setting Auto Sipper

3.3 Measurement

3.3.1 Precautions for Measurement

3.3.2 Concentration Measurement

3.3.3 Wavelength Scan

3.3.4 Time Scan

3.3.5 Monitor Measurement

3.4 Checking of Sipping Amount