horiba Fluorolog-3 User Manual

Summarizes the content of each chapter, providing a roadmap for the manual's structure.

Outlines terms of use, user responsibilities, and limitations of liability for the product.

Presents general safety precautions and symbols used for operating the instrument safely.

Details hazards associated with ultraviolet light exposure and necessary protective measures.

Discusses hazards related to xenon lamps, including visible and infrared radiation.

Specifies the necessary table stability and size for proper system installation.

Outlines recommended temperature and humidity levels for optimal instrument operation.

Details voltage, current, and circuit requirements for powering the instrument.

Provides guidance for scheduling initial installation and subsequent assembly by authorized personnel.

Explains the general operation of the Fluorolog®-3 system and its main components.

Describes fundamental components: monochromators, sample compartment, detector, and accessories.

Details standard system configurations (FL3-11, FL3-12, FL3-21, FL3-22) and their specifications.

Lists available optional components for customizing system configurations and layouts.

Provides step-by-step instructions for powering on the lamp, accessories, and host computer.

Explains how to check system calibration, repeatability, and throughput for routine maintenance.

Details procedures for verifying wavelength calibration of excitation and emission monochromators.

Explains calculation of water-Raman S/N ratio as a measure of instrument sensitivity.

Lists recommended substances for system sensitivity checks and lifetime measurements.

Introduces special FluorEssence™ buttons for recording and presenting data.

Explains how to use the Experiment Menu to select and set up various experiment types.

Details using buttons to reset experiments or rerun previous setups without modifications.

Guides on creating overlay files, 3D scans, batch experiments, and using calibration curves.

Describes using Gemini Alpha for modeling TCSPC lifetime data and predicting lifetime components.

Guides on finding preliminary emission maxima for unknown samples.

Explains how to incorporate dark offset, blank subtraction, and correction factors for accurate signals.

Provides detailed steps for cleaning cuvettes using nitric acid and de-ionized water.

Discusses preparing solutions, small-volume samples, and solid samples for analysis.

Covers precautions with solid sample holders and using filters in the optical path.

Discusses collection methods, optimal wavelengths, G factor, and improving signal-to-noise ratio.

Explains how to remove artifacts and apply correction factors during and after acquisition.

Provides instructions for cleaning the instrument's external case and exhaust filters.

Discusses xenon lamp replacement, required tools, hazards, and adjustment for optimal sensitivity.

Provides instructions for cleaning the lamp housing window and replacing the baffle.

Guides the user through replacing the photomultiplier tube, including safety precautions.

Discusses standard gratings, their specifications, and mirror alignment procedures.

A table listing problems, causes, and remedies for common issues encountered with the system.

Explains how to interpret diagnostic spectra, including lamp and water Raman scans.

Offers advice on steps before contacting service, including website resources and problem duplication.

Explains the need for radiometric correction factors to compensate for wavelength-dependent characteristics.

Describes the 1908 Accessory and the F-3026 Correction Factor Kit.

Details procedures for generating emission correction factors using the 1908 and F-3026 accessories.

Details the process of calculating excitation correction factors using a rhodamine-B solution.

Explains how to use acquired correction files within the Experiment Setup.

Introduces polarized emission measurements for studying molecular rotations and changes.

Provides guidance on installing, removing, and checking polarizer alignment.

Details automatic and manual alignment routines for polarizers, including safety cautions.

Discusses software choices for polarization measurements and applications.

Explains how to measure or input the G factor for polarization wavelength-response correction.

Provides guidance on handling, cleaning, and troubleshooting polarizers.

Introduces the FL-1040 phosphorimeter for measuring long-lived luminescence and phosphorescence.

Explains principles of phosphorescence measurement and the data acquisition sequence.

Explains key parameters and demonstrates applications like decay curves and mixture analysis.

Details the steps for processing phosphorimeter data, including fitting analytical curves.

Provides instructions and warnings for replacing the xenon flash lamp in the phosphorimeter.

Lists common problems, causes, and remedies for the phosphorimeter accessory.

Highlights system calibration, accuracy, and use of data-correction techniques for reproducible spectra.

Demonstrates the system's sensitivity in detecting trace concentrations of fluorescein.

Covers detection of highly scattering samples and quantum-yield calculations.

Discusses synchronous scanning for mixtures and operation in the infrared region.

Explains time-resolved data acquisition and low-temperature scans for enhanced fluorescence.

Covers monitoring kinetic reactions and front-face detection for solid or absorbent samples.

Explains fluorescence polarization for detecting trace biological probes and immunoassay techniques.

Provides information on xenon lamp lifespan and a form for recording usage hours.

Introduces the TRIAX imaging spectrometer and its differences from standard monochromators.

Details electrical power requirements, internal workings, and turret installation.

Explains setting up the Experiment Setup window for CCD acquisition with a TRIAX.

Details adjusting TRIAX parameters in Real Time Control, including grating and mirror settings.

Guides on using System Configuration to set the cooled detector's temperature.

Explains correction-factor file naming conventions and data correction for TRIAX systems.

Lists the technical specifications for the TRIAX 320 spectrograph.

Provides a troubleshooting chart for common problems encountered with the TRIAX spectrograph.

Outlines two methods for upgrading to lifetime capability: frequency domain and TCSPC.

Describes the Fluorolog®-Tau-3 system for frequency domain lifetime measurements.

Explains frequency and time domain lifetime measurement techniques and their parameters.

Details the four available scan types: Lifetime, Lifetime-resolved, Anisotropy-decay, and Time-resolved.

Lists the main components of the Fluorolog®-3 system and its control by a PC.

Details specifications for excitation source, monochromators, sample compartment, and detectors.

Lists the minimum hardware and software requirements for the host computer.

Introduces how the Fluorolog®-3 can be configured with various components and accessories.

Describes the FL-1013 Dewar assembly for low-temperature measurements.

Lists and describes various sample cells for different applications.

Details correction factor kits for calibrating spectral response.

Describes cryostats, CCD detectors, and signal detectors for spectral acquisition.

Details fiber optic mounts for remote sensing and cut-on UV-Visible filters.

Describes cell holders for maintaining samples at constant temperatures with magnetic stirrers.

Details the 1933 holder designed for samples like thin films, powders, and microscope slides.

Describes xenon lamp housings and the autotitration injector accessory.

Details the MicroMax plate reader and L/T-format polarizers for automated measurements.

Describes accessories for studying fluorescence and monitoring fast reactions.

Explains TCSPC upgrades for lifetime capability and the F-1000/1 Temperature Bath.

Guides on connecting the host computer and assembling the spectrofluorometer modules.

Illustrates connectors, SpectrAcq rear panel, and guides on connecting power cables.

Defines terms starting with A, B, and C, related to spectroscopy and instrumentation.

Defines terms starting with C, D, E, and F, related to spectroscopy and instrumentation.

Defines terms starting with F, G, H, I, L, M, related to spectroscopy and instrumentation.

Defines terms starting with R, S, T, related to spectroscopy and instrumentation.

Defines terms starting with T, X, related to spectroscopy and instrumentation.