C-2
SNAP* Total T
4
Test Description
An enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of total T
4
(thyroxine) in canine, feline, and equine patients. With a SNAP Total T
4
Test, you can assess thyroid
function, provide comprehensive one-visit screening for feline hyperthyroidism, presumptive
hyperthyroidism and canine or equine hypothyroidism, as well as monitor response to treatment and
adjust dosages immediately.
Preparing and Running a SNAP Total T
4
Test
Important: Allow the SNAP Total T
4
Test to equilibrate in its sealed foil package at room temperature
for at least 15 minutes. Do not heat. It is not necessary to equilibrate the conjugate solution at room
temperature.
1. Enter the patient information on the IDEXX VetLab* Station (for more information, see the
“Analyzing Samples” chapter of the IDEXX VetLab Station Operator’s Guide).
2. Once the patient’s name appears in the Pending list on the SNAPshot Dx Home screen, tap the
patient’s name and then tap Select. The Identify Sample screen appears.
3. Select Total T
4
as the SNAP Test Type.
4. Select the applicable sample type.
Note: Plasma from the Catalyst* whole blood separator may be used.
5. Tap Next.
6. If you are running a diluted sample, tap the Dilution or arrows to specify the parts diluent
(see the “Total T
4
Dilution Guidelines” section on page C-3).
Note: T
4
dilutions have not been validated for equine samples.
Important: Before performing a dilution, always run an undiluted patient sample first.
Samples should only be diluted when the undiluted sample result is >7.0 μg/dL (90.1 nmol/L).
7. Tap Next and follow the on-screen instructions to properly prepare the sample.
a. Use the purple handheld pipette to transfer 65 μL of sample into a disposable sample tube.
b. Use the 300-μL pipette to transfer 300 μL of conjugate into the same sample tube.
c. Cap the sample tube and gently invert it 4–5 times to mix.
d. Place the sample tube into an available incubator well.
e. Tap to start the incubation timer that corresponds to the well you selected (left or right).
The incubation timer displays with the patient information in the Pending list on the Home
screen (the timer counts down in 10-second intervals).
8. When incubation is complete (the patient information in the Pending list changes from
“Incubating” to “Ready,” the text turns red, and the analyzer beeps), tap to select the patient in
the Pending list.
9. Tap Next and follow the on-screen instructions to properly prepare the SNAP test:
a. Place the device on a horizontal surface.
b. Add the entire contents of the sample tube to the sample well, being careful not to
splash the contents outside of the sample well. The sample will flow across the result
window, reaching the activation circle in 30–60 seconds. Some sample may remain in
the sample well.
c. When color FIRST appears in the activation circle, press the activator firmly until it is
flush with the device body.
SNAP Test Procedures
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