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Implen NanoPhotometer User Manual

Implen NanoPhotometer
70 pages
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NanoPhotometer
®
P-Class
User Manual
P 300 / P 330 / P 360
Version 2.1
P 360
P 300
P 330

Table of Contents

Questions and Answers:

Implen NanoPhotometer Specifications

General IconGeneral
CategorySpectrophotometer
Light SourceXenon flash lamp
Measurement Time< 5 seconds
Detector2048 element CCD array
ConnectivityUSB, Ethernet, Wi-Fi
Absorbance Range0.02 - 300 A (depending on path length)
Display7" Touchscreen

Summary

Essential Safety Notes

Unpacking, Positioning and Installation

Instructions for unpacking, positioning, and installing the instrument safely.

Introduction

Your Spectrophotometer

Overview of the NanoPhotometer P-Class spectrophotometer, its CCD array detector, and operating system.

Sample Handling Tips

Guidance on sample handling, cell insertion, volume, and using test tubes for measurements.

Keypad and Display for NanoPhotometer P 300

Description and function of the keypad and LCD display for the P 300 model.

Keypad and Display for NanoPhotometer P 330/P 360

Description and function of the keypad and LCD display for the P 330/P 360 models.

Menu/Options

Details on menu options for P 300 and P 330/P 360 models after measurements.

NanoPhotometer P-Class Submicroliter Cell

Technical Instructions

Step-by-step guide for inserting the submicroliter cell and preparing samples for measurement.

Software Instructions

Procedure for using software for NanoVolume and Cuvette applications with the submicroliter cell.

NanoVolume and Cuvette Applications

DNA, RNA, and Oligonucleotide Characterization

General information on quantifying and checking purity of DNA, RNA, and oligonucleotides.

Protein Determination

General information and methods for protein determination using UV absorption and colorimetric assays.

Bacterial Cell Culture Measurement (OD600)

Guidance on monitoring bacterial growth using optical density measurements at 600 nm.

Functions

Single Wavelength - Absorbance and %T

Measures light passed through a sample relative to a reference for absorbance or % transmission.

Concentration Measurement

Measures sample absorbance and calculates concentration using a known factor or standard.

Wavescan

Obtains an absorption spectrum to identify peak height and position for simple spectral analysis.

Kinetics

Monitors absorbance change over time at a fixed wavelength for kinetic studies.

Standard Curve

Constructs multi-point calibration curves from known standards to quantify unknown samples.

Multiple Wavelength

Performs up to 5 absorbance measurements on the same sample at different wavelengths.

Absorbance Ratio

Measures absorbance at two wavelengths and calculates the ratio, corrected for background.

User Methods

Managing User Methods

Options for renaming, locking, unlocking folders, and managing methods via SD card.

Utilities

Date and Time Settings

Procedure for setting the instrument's date and time.

Regional Settings

Configures number format, such as decimal point style, for regional preferences.

Output Options and Printer

Settings for data output to PC, SD Memory Card, or the built-in printer.

Preferences

User preferences for games, screen layout, history, standby mode, and baseline compensation.

Contrast and Brightness Adjustment

Adjusts display brightness and contrast for optimal visibility under local conditions.

About the Instrument

Displays the instrument's serial number, software version, and manufacturer website.

Maintenance

Maintenance-free Technology

The instrument is maintenance-free; secondary standards are available for performance verification.

Lamp Replacement

Xenon lamp replacement should be done by a service engineer after several years of use.

Mixer Replacement

Mixer replacement should be done by a service engineer after several years of use.

Exchange of the Gaiter

Instructions for replacing a damaged gaiter, item number S-330360-G.

Cleaning and General Care

Guidelines for external cleaning, surface care, and handling hazardous samples.

Error Messages and Troubleshooting

Lists common error messages, their explanations, and solutions for troubleshooting.

Accessories

Specifications and Warranty

Solvent Compatibility

Appendix

Nucleic Acid Quantification

Determines nucleic acid concentration using absorbance at 260 nm and the Lambert-Beer equation.

Nucleic Acid Fluorescent Dye Incorporation

Determines nucleic acid and dye concentrations using fluorescence and dye correction.

Protein Quantification

Determines protein concentration using absorbance at 280 nm and the Lambert-Beer equation.

Protein Fluorescent Dye Incorporation

Determines protein and dye concentrations after labelling, with dye correction.

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