Implen NanoPhotometer User Manual

NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 11 / 70
Operation Limitations: Do not autoclave the unit! Do not use an ultrasound bath to clean! Do not drop in water or
solvent bath. The unit is water resistant, but not water proof!
3.2 Software instructions
The NanoVolume Applications and Cuvette Applications are very similar concerning the analysis of dsDNA, ssDNA, RNA,
Oligonucleotides, protein UV and protein dye analysis. This section describes the specific features which have to be
considered using the NanoPhotometer
®
P-Class Submicroliter Cell. For general information please follow the detailed
instructions under Nanovolume Applications and Cuvette Applications.
The procedure is as follows:
Exemplary Parameter Screen Parameter Screen
Step 1 Press 1 to select NanoVolume Applications folder
Step 2 Press 1 to select Nucleic Acids folder OR 2 to select
Protein folder.
Step 3 Select the method you want to use by pressing the
corresponding number.
Step 4 Select the Lid Factor using the left and right arrows.
Lid
Sample volume
Pathlength
Dilution
5 (optional)
3.5 – 5 µl
2 mm
1:5
10 (optional for P 300)
1 – 3 µl
1 mm
1:10
50
0.3 – 2 µl
0.2 mm
1:50
100 (optional)
0.3 – 2 µl
0.1 mm
1:100
250 (optional)
0.3 – 2 µl
0.04 mm
1:250
Step 5 Select subsequent parameters and specifications as
described under 4. Nanovolume Applications and
Cuvette Applications.
After the selections are confirmed the results screen displays in
top left corner the chosen Lid and the required sample volume.

Implen NanoPhotometer Specifications

General

Category	Spectrophotometer
Light Source	Xenon flash lamp
Measurement Time	< 5 seconds
Detector	2048 element CCD array
Connectivity	USB, Ethernet, Wi-Fi
Absorbance Range	0.02 - 300 A (depending on path length)
Display	7" Touchscreen