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Implen P 300 User Manual

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telephone: + 49-89-726 3718 0 Fax. + 49-89-726 3718 54 Email: info@implen.de www.implen.de
NanoPhotometer
®
P-Class
User Manual
P 300 / P 330 / P 360
Version 2.1
P 360
P 300
P 330

Table of Contents

Questions and Answers:

Implen P 300 Specifications

General IconGeneral
BrandImplen
ModelP 300
CategoryMeasuring Instruments
LanguageEnglish

Summary

Declaration of Conformity

Conformity to EU Directives

Lists EU directives for compliance.

Compliance with Technical Standards

Details harmonized standards followed.

Essential Safety Notes

Installation and Environmental Requirements

Guidelines for proper setup and operating conditions.

P300 Keypad and Display

P330/P360 Keypad and Display

Submicroliter Cell Usage

Technical Instructions for Submicroliter Cell

Step-by-step guide for using the submicroliter cell.

NanoVolume and Cuvette Applications Overview

Nucleic Acid Analysis (DNA, RNA, Oligo)

Introduces nucleic acid quantification and purity checks.

Analysis of dsDNA, ssDNA, and RNA

Parameter Setup for Nucleic Acid Analysis

Steps to configure parameters for dsDNA, ssDNA, RNA.

Analysis of Oligonucleotides

Parameter Setup for Oligonucleotide Analysis

Steps to configure parameters for oligonucleotides.

Nucleic Acid Dye Incorporation Methods

Parameter Setup for Dye Incorporation

Steps to configure parameters for dye incorporation.

Protein Determination Methods

Protein Measurement at 280 nm

Method for quantifying proteins using absorbance at 280 nm.

Colorimetric Protein Assays

Overview of Bradford, Biuret, BCA, Lowry assays.

Protein UV Method

Parameter Setup for Protein UV Method

Steps to configure parameters for protein UV measurement.

Protein UV Dye Method

Parameter Setup for Protein UV Dye Method

Steps to configure parameters for protein UV dye measurement.

BCA Assay Procedure

BCA Parameter Screen Setup

Initial setup for the BCA assay.

BCA Calibration Process

BCA Manual Calibration and Results

Bradford Assay Procedure

Bradford Parameter Screen Setup

Initial setup for the Bradford assay.

Bradford Calibration Process

Bradford Manual Calibration and Results

Lowry Assay Procedure

Lowry Parameter Screen Setup

Initial setup for the Lowry assay.

Lowry Calibration Process

Lowry Manual Calibration and Results

Biuret Assay Procedure

Biuret Parameter Screen Setup

Initial setup for the Biuret assay.

Biuret Calibration Process

Biuret Manual Calibration and Results

Bacterial Cell Culture Measurement (OD600)

General Information on OD600 Measurements

Principles and factors affecting OD600 readings.

Analysis of Bacterial Growth (OD600)

Parameter Setup for Bacterial Growth Analysis

Steps to configure parameters for bacterial growth monitoring.

Single Wavelength Measurements (Abs/%T)

Parameter Setup for Single Wavelength

Steps to configure parameters for single wavelength measurements.

Concentration Measurements

Parameter Setup for Concentration Measurement

Steps to configure parameters for concentration determination.

Concentration Measurements in Standard Mode

Wavescan Operation

Parameter Setup for Wavescan

Steps to configure parameters for spectral scanning.

Peak Detection in Wavescan

Kinetics Measurements

Parameter Setup for Kinetics Assays

Configuring parameters for time-dependent measurements.

Standard Curve Creation

Standard Curve Parameter Setup

Configuring parameters for generating calibration curves.

Standard Curve Calibration Process

Steps for performing calibration measurements.

Standard Curve Calibration Process

Standard Curve Manual Calibration & Results

Multiple Wavelength Measurements

Parameter Setup for Multiple Wavelengths

Steps to configure parameters for multiple wavelength measurements.

Absorbance Ratio Measurements

Parameter Setup for Absorbance Ratio

Steps to configure parameters for absorbance ratio determination.

User Methods and Saved Procedures

Managing User Methods (Folders, Locking)

Organizing, protecting, and renaming saved methods.

Date, Time, and Regional Settings

Output Options / Printer (P300)

Data Output Configuration

User Preferences and Display Settings

Instrument Maintenance Information

Maintenance-Free Technology Overview

Information on the lack of routine maintenance.

Lamp and Mixer Replacement Notes

Guidance on component lifespan and replacement.

Error Messages and Troubleshooting

Common Error Messages and Solutions

Detailed list of error messages and their resolutions.

Troubleshooting Common Symptoms

Troubleshooting Instrument Issues

Solutions for common operational problems.

Technical Specifications and Warranty

Solvent Compatibility

Appendix: Nucleic Acid Calculations

Appendix: Protein Calculations

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