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Implen P 300 - User Manual

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telephone: + 49-89-726 3718 0 Fax. + 49-89-726 3718 54 Email: info@implen.de www.implen.de
NanoPhotometer
®
P-Class
User Manual
P 300 / P 330 / P 360
Version 2.1
P 360
P 300
P 330

Table of Contents

Questions and Answers

  • A
    aliciasmithAug 8, 2025
    What to do if the Implen P 300 Measuring Instruments thermal paper printer prints a lot of paper?
    • R
      Richard WardAug 9, 2025
      If the Implen Measuring Instruments thermal printer is printing a lot of paper with a normal sound but no error message, the thermal printer paper was probably put in the wrong way. Check the correct position of the printer paper.
  • J
    jacksonmariaAug 14, 2025
    Why does my Implen P 300 instrument switch off during measurement?
    • M
      mrichardsonAug 14, 2025
      If your Implen Measuring Instruments instrument intermittently switches off during measurement, it could be due to a faulty or loose power input connection. Check the voltage output of the power supply.
  • K
    Kim Adams MDAug 20, 2025
    Why does my Implen P 300 instrument switch off after calibration?
    • E
      elizabethgriffithAug 20, 2025
      If the Implen Measuring Instruments instrument is switching off after calibration, it might be because you are holding the ON/OFF button too long. Avoid holding the ON/OFF button for an extended period.
  • A
    Alexis GarzaAug 27, 2025
    What to do if Implen Measuring Instruments thermal paper printer displays random numbers?
    • D
      Deanna HarrisonAug 27, 2025
      If the Implen Measuring Instruments thermal paper printer displays random or endless numbers after a paper exchange, it's possible that the paper roll was changed while the instrument was still in one of its programs. Move to the main menu before exchanging the paper roll. Then, switch the device off, disconnect the power supply, and reconnect it after about 5 minutes. A firmware update (version 2.4.2) is also recommended.
  • G
    Gabrielle PerkinsSep 2, 2025
    How to fix Implen P 300 failing start up calibration?
    • D
      Deborah PonceSep 2, 2025
      If the Implen Measuring Instruments instrument is failing to start up calibration, check that the correct power supply (18 V, 1.2 A) is being used and ensure that the connector is pushed in fully.

Summary

Declaration of Conformity

Conformity to EU Directives

Lists EU directives for compliance.

Compliance with Technical Standards

Details harmonized standards followed.

Essential Safety Notes

Installation and Environmental Requirements

Guidelines for proper setup and operating conditions.

P300 Keypad and Display

P330;P360 Keypad and Display

Submicroliter Cell Usage

Technical Instructions for Submicroliter Cell

Step-by-step guide for using the submicroliter cell.

NanoVolume and Cuvette Applications Overview

Nucleic Acid Analysis (DNA, RNA, Oligo)

Introduces nucleic acid quantification and purity checks.

Analysis of dsDNA, ssDNA, and RNA

Parameter Setup for Nucleic Acid Analysis

Steps to configure parameters for dsDNA, ssDNA, RNA.

Analysis of Oligonucleotides

Parameter Setup for Oligonucleotide Analysis

Steps to configure parameters for oligonucleotides.

Nucleic Acid Dye Incorporation Methods

Parameter Setup for Dye Incorporation

Steps to configure parameters for dye incorporation.

Protein Determination Methods

Protein Measurement at 280 nm

Method for quantifying proteins using absorbance at 280 nm.

Colorimetric Protein Assays

Overview of Bradford, Biuret, BCA, Lowry assays.

Protein UV Method

Parameter Setup for Protein UV Method

Steps to configure parameters for protein UV measurement.

Protein UV Dye Method

Parameter Setup for Protein UV Dye Method

Steps to configure parameters for protein UV dye measurement.

BCA Assay Procedure

BCA Parameter Screen Setup

Initial setup for the BCA assay.

BCA Calibration Process

BCA Manual Calibration and Results

Bradford Assay Procedure

Bradford Parameter Screen Setup

Initial setup for the Bradford assay.

Bradford Calibration Process

Bradford Manual Calibration and Results

Lowry Assay Procedure

Lowry Parameter Screen Setup

Initial setup for the Lowry assay.

Lowry Calibration Process

Lowry Manual Calibration and Results

Biuret Assay Procedure

Biuret Parameter Screen Setup

Initial setup for the Biuret assay.

Biuret Calibration Process

Biuret Manual Calibration and Results

Bacterial Cell Culture Measurement (OD600)

General Information on OD600 Measurements

Principles and factors affecting OD600 readings.

Analysis of Bacterial Growth (OD600)

Parameter Setup for Bacterial Growth Analysis

Steps to configure parameters for bacterial growth monitoring.

Single Wavelength Measurements (Abs;%T)

Parameter Setup for Single Wavelength

Steps to configure parameters for single wavelength measurements.

Concentration Measurements

Parameter Setup for Concentration Measurement

Steps to configure parameters for concentration determination.

Concentration Measurements in Standard Mode

Wavescan Operation

Parameter Setup for Wavescan

Steps to configure parameters for spectral scanning.

Peak Detection in Wavescan

Kinetics Measurements

Parameter Setup for Kinetics Assays

Configuring parameters for time-dependent measurements.

Standard Curve Creation

Standard Curve Parameter Setup

Configuring parameters for generating calibration curves.

Standard Curve Calibration Process

Steps for performing calibration measurements.

Standard Curve Calibration Process

Standard Curve Manual Calibration & Results

Multiple Wavelength Measurements

Parameter Setup for Multiple Wavelengths

Steps to configure parameters for multiple wavelength measurements.

Absorbance Ratio Measurements

Parameter Setup for Absorbance Ratio

Steps to configure parameters for absorbance ratio determination.

User Methods and Saved Procedures

Managing User Methods (Folders, Locking)

Organizing, protecting, and renaming saved methods.

Date, Time, and Regional Settings

Output Options; Printer (P300)

Data Output Configuration

User Preferences and Display Settings

Instrument Maintenance Information

Maintenance-Free Technology Overview

Information on the lack of routine maintenance.

Lamp and Mixer Replacement Notes

Guidance on component lifespan and replacement.

Error Messages and Troubleshooting

Common Error Messages and Solutions

Detailed list of error messages and their resolutions.

Troubleshooting Common Symptoms

Troubleshooting Instrument Issues

Solutions for common operational problems.

Technical Specifications and Warranty

Solvent Compatibility

Appendix: Nucleic Acid Calculations

Appendix: Protein Calculations

Implen P 300 Specifications

General IconGeneral
BrandImplen
ModelP 300
CategoryMeasuring Instruments
LanguageEnglish

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