NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 3 / 70
TABLE OF CONTENTS
1. ESSENTIAL SAFETY NOTES ............................................................................................................................................... 5
Unpacking, Positioning and Installation ..................................................................................................... 5
2. INTRODUCTION .................................................................................................................................................................. 6
2.1 Your spectrophotometer ..................................................................................................................... 6
2.2 Sample handling tips .......................................................................................................................... 6
2.3 Keypad and display for NanoPhotometer
®
P 300 ............................................................................. 7
2.4 Keypad and display for NanoPhotometer
®
P 330 /P 360 ................................................................. 8
2.5 Menu/Options ....................................................................................................................................... 9
3. THE NANOPHOTOMETER
®
P-CLASS SUBMICROLITER CELL ........................................................................................ 10
3.1 Technical instructions ...................................................................................................................... 10
3.2 Software instructions ........................................................................................................................ 11
4. NANOVOLUME APPLICATIONS AND CUVETTE APPLICATIONS .................................................................................... 13
4.1 Characterization of DNA, RNA and Oligonucleotides ................................................................... 13
4.1.1 General Information ................................................................................................................ 13
4.1.2 Analysis of dsDNA, ssDNA and RNA ..................................................................................... 15
4.1.3 Analysis of Oligonucleotides ................................................................................................... 16
4.1.4 Dye incorporation for dsDNA, ssDNA, RNA and Oligonucleotides ......................................... 17
4.2 Protein Determination ....................................................................................................................... 19
4.2.1 General Information ................................................................................................................ 19
4.2.2 Protein UV Method .................................................................................................................. 20
4.2.3 Protein UV Dye Method .......................................................................................................... 22
7.3.4 BCA Assay .............................................................................................................................. 24
7.3.5 Bradford Assay ........................................................................................................................ 27
7.3.6 Lowry Assay ............................................................................................................................ 30
7.3.7 Biuret Assay ............................................................................................................................ 33
4.3 Bacterial Cell Culture Measurement (OD600) ................................................................................. 36
4.3.1 General Information ................................................................................................................ 36
4.3.2 Analysis of Bacterial Growth ................................................................................................... 37
5. FUNCTIONS ...................................................................................................................................................................... 38
5.1 Single Wavelength – Abs and %T .................................................................................................... 39
5.2 Concentration .................................................................................................................................... 41
5.3 Wavescan ........................................................................................................................................... 43
5.4 Kinetics ............................................................................................................................................... 46
5.5 Standard Curve .................................................................................................................................. 48
5.6 Multiple Wavelength .......................................................................................................................... 51
5.7 Absorbance Ratio .............................................................................................................................. 53
6. USER METHODS .............................................................................................................................................................. 55
7. UTILITIES .......................................................................................................................................................................... 56
7.1 Date and Time .................................................................................................................................... 57
7.2 Regional .............................................................................................................................................. 57
7.3 Output Options / Printer .................................................................................................................... 57
7.3.1 NanoPhotometer
®
P 300 ......................................................................................................... 57
7.3.2 NanoPhotometer
®
P 330 /P 360 ............................................................................................. 58
7.3.3 Loading / changing the printer paper ...................................................................................... 59
7.4 Preferences ........................................................................................................................................ 60
7.5 Contrast .............................................................................................................................................. 60
7.6 About .................................................................................................................................................. 60
8. MAINTENANCE ................................................................................................................................................................. 61
8.1 Maintenance-free Technology .......................................................................................................... 61
8.2 Lamp Replacement ............................................................................................................................ 61
8.3 Mixer replacement ............................................................................................................................. 61
8.4 Exchange of the gaiter ...................................................................................................................... 61
8.5 Cleaning and general care of the instrument ................................................................................. 62
8.6 Error messages ................................................................................................................................. 63
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