NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 53 / 70
5.7 Absorbance Ratio
This makes simple absorbance ratio measurements on samples, measuring the amount of light that has passed
through a sample relative to a blank (this can be air) at two wavelengths.
The procedure is as follows:
Parameter Screen
Parameter Screen
Step 1 Press 3 to select Functions.
Step 2 Press 7 to select Absorbance Ratio
Step 3 Enter the first Wavelength by using the keypad numbers
or the left and right arrows.
Step 4 Enter the second Wavelength as above.
Step 5 Select whether a Background correction is applied to
both wavelengths 1 and 2 using the left and right arrows.
Step 6 (If background correction is On) Enter the third
Wavelength, from which the background correction will
be obtained.
Step 7 Press Next to enter the next screen OR press Cancel
to return to the Functions folder.
Step 8 (Dilution Factor known) Enter a Dilution factor by using
the keypad numbers within the range 1.00 – 9,999.
Step 9 (Calculate Dilution Factor) Press the Menu/Options key.
Enter the Volume of the sample (range 0.01 – 9,999),
using the keypad numbers. Enter the volume of Diluent
(range 0.01-9,999) by using the keypad numbers.
Step 10 Press OK to calculate the dilution factor and return to
the Parameters screen OR press Cancel to cancel
selections.
Step 11 Select units of measurement, using left and right arrows.
Options are: µg/ml, ng/µl, µg/µl.
Step 12 Enter the factor using the keypad numbers (Range 0.001
to 9,999).
Step 13 Press OK to enter the results screen OR Cancel to
return to the Functions folder.
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