Implen P 300 User Manual

NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 10 / 70
3. THE NANOPHOTOMETER
®
P-CLASS SUBMICROLITER CELL
With its innovative optical pathway the cell is designed for optimum measurement results with submicroliter samples
ranging from 0.3 µl up to 5 µl of undiluted sample. Due to a pathlength of 0.04 mm, 0.1 mm, 0.2 mm, 1 mm and 2
mm the cell is offering an automatic dilution of 1/250, 1/100, 1/50, 1/10 and 1/5 in comparison to a standard
cuvette measurement. Because the measurements are processed with undiluted samples, the reproducibility of the
results is extremely high. If desired, samples can be retrieved after the measurement for further processing. The
NanoPhotometer
®
P-Class Submicroliter Cell can be used for all UV/Vis analysis utilizing the wavelength range of
190 nm to 1,100 nm.
The NanoPhotometer
®
P-Class Submicroliter Cell is delivered for version P 300 with one lid
with a pathlength of 0.2 mm (Lid 50), for version P 330 with two lids pathlength 0.2 mm
(Lid 50) and 1 mm (Lid 10) and for version P 360 with three lids pathlength 0.04 mm (Lid
250), 0.2 mm (Lid 50) and 0.1 mm (Lid 10). Lid 5 (2 mm pathlength), Lid 100 (0.1 mm
pathlength) and Lid 250 (0.04 mm) can be ordered optionally. The dilution factor (lid
factor) is printed on the lid. Please make sure that you use the appropriate lid for your
sample.
3.1 Technical instructions
Step 1
Insert the NanoPhotometer
®
P-Class Submicroliter Cell into the cell
holder with the cell windows facing the light beam. We recommend
facing the Implen logo to the front. The light beam is directed from
RIGHT to LEFT as indicated with small arrows. Insert the
NanoPhotometer
®
P-Class Submicroliter Cell always in the same
direction.
Step 2
Use the integrated vortexer (P 330 / P 360 only) to mix your sample well
to achieve an accurate homogeneity of the sample.
Step 3
Pipette the appropriate sample volume onto the centre of the measuring
window. Warning!! Do not overfill the well.
Lid
Sample volume
Pathlength
Dilution
5 (optional)
3.5 – 5 µl
2 mm
1:5
10 (optional for P300)
1 – 3 µl
1 mm
1:10
50
0.3 – 2 µl
0.2 mm
1:50
100 (optional)
0.3 – 2 µl
0.1 mm
1:100
250 (optional)
0.3 – 2 µl
0.04 mm
1:250
Step 4
Make sure that for the measurements the lid fits exactly onto the
positioning supports mounted to the body of the cell. Take
measurement. Remember to consider the lid factor in your instrument
software. Please refer to the NanoPhotometer
®
P-Class User Manual for
detailed information.
Step 4
Take the lid off and retrieve the sample with a pipette for further
applications if desired. Remove sample residues from the measurement
window and the mirror in the lid. Clean the measurement window and
mirror in the lid well with a slightly wet fluff-free tissue. Use water, 70%
ethanol or isopropanol. Do not use aggressive solvents like strong acids
or bases or organic solvents at any time.
Important Note: Residual fluffs must be removed for optimum
performance
Your cell is ready for the next sample.

Implen P 300 Specifications

General

Brand	Implen
Model	P 300
Category	Measuring Instruments
Language	English