NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 30 / 70
7.3.6 Lowry Assay
The colorimetric Lowry assay is not recommended with the Submicroliter Cell. Please use Cuvette Applications.
The procedure is as follows:
Parameter Screen
Standards Screen
Parameter Screen
Step 1 Press 2 to select Cuvette folder.
Step 2 Press 2 to select Protein folder.
Step 3 Press 4 to select Lowry mode.
Step 4 The default Wavelength setting is 750 nm.
Step 5 Enter the number of Standard concentration points (1-9)
to be used in the curve using the keypad numbers or left
and right arrows.
Step 6 Units: The user can enter a text string up to 8 characters
long. To access a list of pre-defined units press the
Menu/Options key and then use the left/right arrows
(µg/ml, µg/µl, pmol/µl, mg/ml, mmol/l, µmol/l, g/l,
mg/l, µg/l, U/l, %, ppm, ppb, conc or none). These units
can also be edited once OK is pressed. This screen also
allows the number of displayed Decimal Points (DP) to
be selected, from 0 to 2. Note that the result will always
be fixed to 5 significant figures regardless of how many
decimal points are selected (so 98768.2 will display as
98768 even with 1 decimal point selected). Press OK
to store the chosen parameters OR Cancel
.
Step 7 Press Next to enter the next screen.
Step 8 Select the Calibration mode, either Standards (measure
prepared standards), Manual (keypad data entry) or
New Standards (previous values are blanked, new
standard can be measured).
Step 9 (if Standards selected) Select the number of Replicates
using the left and right arrows. This determines the
number of standards to be measured and averaged at
each standard concentration point. Can be Off (1), 2 or
3.
Step 10 Press Next to enter the Standards screen OR press
Cancel to cancel selections and return to the Protein
folder.
Standards Screen
Step 11 Enter the concentration values by using the keypad
numbers and the up and down arrows to move between
the different standard boxes. Range 0.001 to 9,999. C
button backspaces and clears the last digit entered.
Step 12 Press Next to enter the Calibration screen. If there
are duplicate or non-monotonic (increasing) entries the
unit will beep and highlight the incorrect entry. OR press
Back to return to the Parameter screen.
To Next Page
Loading...
Need help?
General
