Jasco J-715 User Manual

Manual for CD spectrometer
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Go through the “Switch ON procedure” only 1-2 hours before using it!
- Turn on the N
2
ow on the “wall column”. Only use the black wheel (not the pressure-reducing
valve).
- Turn on the “red box” (behind the box).
- Adjust the N
2
ow on the little black wheel to 12-20l/min.
- Turn on the cooling water
N
2
ow is 12-20l/min and the cooling water is on? If yes go ahead:
- Tun on lamps of the power supply: POWER1, then POWER 2
- Switch on the temperature controller (PTC-348MI) and press “Start”.
- Start the computer and start the software on the desktop.
Sample Preparation:
1) Prepare your sample in a volumetric ask, don’t ll it into a cuvette.
Check on the UV/vis spectrometer, wheter you are in the right concentration range.
- Strong chromophores: c ≈ 10
-5
M. If necessary, start diluting it after a rst quick measurement.
- Weak chromophores: c ≈ 10
-4
M. If necessary, start diluting it after a rst quick measurement.
- GENERAL RULE: your absorbance band of interest should be at 0.1-0.8 E, ideally at around
0.2-0.4 E.
2) Fill your cuvette with pure solvent.
Start your measurement: (after the machine warmed up for 1-2 hours)
- Choose “Setup”, then setup some parameters which you nd in the submenus.
- Insert your cuvette with pure solvent into the machine and record a Baseline. Save it.
- Remove cuvette, empty it, blow it out with N
2
and ll your sample solution. Put it into the
machine.
- Measure your sample.
- Measure as many samples as your want, all will be automatically be corrected your baseline
le.
Turn off the machine:
- close the Specta Manager software shut down the computer
- turn off the PTC-348WI turn off Power 2 switch
- turn off Power 1 switch
- purge instrument with N
2
for 5 minutes after shutdown before turning off the nitrogen ow.

Jasco J-715 Specifications

General

Light Source	150W Xenon lamp
Detector	Photomultiplier tube
Model	J-715
Wavelength Range	163-900 nm
Measurement Modes	CD, Absorbance, Fluorescence
Temperature Control	Optional Peltier temperature controller
Software	Spectra Manager

Summary

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Switch ON Procedure

N2 Flow and Cooling Water Check

Verify N2 flow is 12-20l/min and cooling water is on before proceeding with instrument startup.

Power Supply and Temperature Controller Startup

Turn on POWER1 and POWER 2, then start the temperature controller (PTC-348MI) and computer.

Sample Preparation

Prepare Sample in Volumetric Flask

Prepare sample in a flask, check concentration with UV/vis spectrometer, dilute if needed.

Fill Cuvette with Solvent

Fill the cuvette with pure solvent before starting the measurement process.

Start Measurement

Setup and Baseline Recording

Choose 'Setup', configure parameters, insert solvent cuvette, and record a Baseline.

Sample Measurement

Replace solvent cuvette with sample, measure sample, and record multiple samples.

Turn Off Machine

Software and Computer Shutdown

Close Specta Manager software and shut down the computer.

Hardware Power Down

Turn off the PTC-348WI, Power 2 switch, and Power 1 switch.

Nitrogen Purge

Purge the instrument with N2 for 5 minutes after shutdown before turning off nitrogen flow.