Jasco V-550 - User Manual

Other manuals for Jasco V-550

Hardware/Function Manual48 pages

Questions and Answers

G
Gabriel MitchellAug 19, 2025
Why is noise high in Jasco Measuring Instruments?
- J
  Jennifer LewisAug 19, 2025
  High noise in Jasco Measuring Instruments can occur for several reasons: * The light may not be correctly hitting the center of the entrance slit; if this is the case, adjust the light source mirror. * The spectral bandwidth might be too small; try increasing it. * The absorption of the solvent on the reference beam side may be too high; replace the solvent with a more suitable one. * The lamp may have deteriorated; replace it with a new one.
N
nicolebeckerAug 21, 2025
What to do if Jasco Measuring Instrument does not operate?
- K
  kelly37Aug 21, 2025
  If your Jasco Measuring Instrument isn't operating, first ensure the power cable is securely plugged into the outlet. If it's disconnected, replug it. If the issue persists, remove the power cable and check the fuse in the main unit. Replace the fuse if it's blown. If the fuse blows immediately after replacement, it indicates a fault in the electrical system.
A
Anthony RodriguezAug 23, 2025
What to do if the light source changeover from WI to D causes a baseline step height of more than 1% in Jasco V-550?
- D
  Danielle CastroAug 23, 2025
  If you observe a baseline step height exceeding 1% when switching the light source from WI to D in your Jasco Measuring Instruments, there are several potential causes: 1. The baseline correction might be set to “Off”. Check this setting and set it to “On” if necessary. 2. The light source mirror might be poorly adjusted. Readjust the mirror. 3. The sample chamber might not be securely fitted, causing the beam to hit the cell holder. Ensure the sample chamber is correctly positioned.
M
Melissa ThompsonAug 26, 2025
Why is the baseline curved greatly in excess of 1% on Jasco Measuring Instruments?
- K
  Katrina RiceAug 26, 2025
  A greatly curved baseline (in excess of 1%) in Jasco Measuring Instruments may be due to the baseline correction being set to “Off”. Check this setting and set it to “On”.
M
Michelle StoneAug 27, 2025
How to fix unstabilized performance at wavelengths shorter than 320 nm on Jasco Measuring Instruments?
- M
  Mark Clark DDSAug 27, 2025
  If the performance at wavelengths shorter than 320 nm is not stabilized on your Jasco Measuring Instruments, the D lamp might be “Off”. Set the D lamp to “On.”
A
awrightAug 29, 2025
How to fix unstabilized performance at wavelengths longer than 400 nm on Jasco Measuring Instruments?
- R
  Russell KingAug 29, 2025
  If the performance at wavelengths longer than 400 nm is not stabilized on your Jasco Measuring Instruments, the WI lamp might be “Off”. Set the WI lamp to “On.”
T
Timothy NelsonAug 31, 2025
What to do if WI lamp does not light in Jasco V-550?
- D
  Daniel AndersonAug 31, 2025
  If the WI lamp does not light in your Jasco Measuring Instruments, check if the lamp has burnt out. If it has, replace it with a new one.
K
Kyle WashingtonSep 3, 2025
What to do if Jasco V-550 Measuring Instruments do not operate at all?
- G
  geraldglassSep 3, 2025
  If the Jasco Measuring Instruments do not operate, first, check if the power cable is securely plugged into the outlet; if disconnected, replug it. If the issue persists, remove the power cable and check the fuse in the main unit. If the fuse is blown, replace it. If the fuse blows immediately after replacement, the electrical system is faulty.
W
William LongSep 5, 2025
Why is there high noise in Jasco Measuring Instruments?
- S
  Sandra JenningsSep 5, 2025
  High noise in Jasco Measuring Instruments can occur because the light is not correctly incident upon the center of the entrance slit. If this is the case, you should adjust the light source mirror. Also, high noise may be caused by a spectral bandwidth that is too small, so try increasing the spectral bandwidth. Another reason can be that the absorption of the solvent placed on the reference beam side is too high, in this case replace the solvent with a suitable one. Finally, high noise may appear because the lamp has deteriorated, you should replace it with a new one.
N
ntaylorSep 7, 2025
What to do if the WI lamp does not light in Jasco V-550?
- V
  Veronica AlvarezSep 7, 2025
  If the WI lamp does not light in Jasco Measuring Instruments, check if the WI lamp has burnt out. If it has, replace the lamp with a new one.

Summary

Safety Considerations

(1) Safety symbols

Defines signal words like WARNING, CAUTION, and Note for hazard identification.

(2) Warning Label

Details on instrument warning labels for fuse and ground connections.

(2) Warning for carrying

Installation Requirements

Limited Warranty

2. Starting and Exiting Programs

2.1 Startup

Procedures for powering on the instrument, PC, and software.

2.1.1 Turning ON the spectrophotometer

2.1.2 PC and Windows startup

2.1.3 [Spectra Manager] startup

2.2 Exiting Programs

2.2.1 Exiting measurement or spectra analysis program

2.2.2 PC and spectrophotometer shutdown

3. Quantitative Analysis and Spectrum Measurement

3.1 Quantitative Analysis Introduction

Overview of quantitative analysis and spectrum measurement procedures.

3.1.1 Quantitative analysis program overview

Features of the quantitative analysis program, including analysis methods.

3.1.1.1 Quantitative analysis program

3.1.1.2 Quantitative analysis operation

3.1.3 Calibration curve creation

Detailed steps for creating calibration curves for analysis.

3.1.4 Calibration curve modification

Procedures for adjusting or re-measuring calibration curve data.

3.1.5 Saving quantitative analysis method

3.1.6 Unknown sample measurement

Steps for measuring unknown samples and calculating concentrations.

3.2 Spectrum Measurement

3.2.1 Procedural overview

Overview of measuring sample spectra, baseline, and saving data.

3.2.2 Spectrum measurement program startup

3.2.3 Setting measurement parameters

Configuring measurement settings like mode, range, and speed.

3.2.4 Setting the baseline (or Measurement)

Process for establishing a baseline for accurate spectral measurements.

3.2.5 Sample measurement

How to measure sample spectra after setting up the baseline.

3.2.6 Spectrum save

3.2.8 Instrument shutdown

4. [Quantitative Analysis]

Quantitative Analysis window

Describes the main windows within the Quantitative Analysis program.

4.1 File menu

4.2 Method menu

4.3 Measurement menu

5. [Spectrum Measurement]

5.1 Measurement menu

Contains options to start measurement, set parameters, and manage baseline.

5.1.1 [Start]

Initiates the spectrum measurement process.

5.1.2 Parameter...

Setting up measurement parameters and auto-save options.

5.1.3 Baseline...

5.1.4 Move Wave length...

5.1.5 [Auto Zero]

6. [Time Course Measurement]

6.1 Measurement menu

Options for starting time course measurement and setting parameters.

6.1.1 [Start]

Initiates the time course measurement process.

6.1.2 Parameter...

7. [Fixed Wavelength Measurement]

7.1 Measurement menu

7.1.1 [Start]

7.1.2 Blank ([Blank]) Blank Correct] ([Correct])

7.1.3 Parameter...

7.1.4 [Goto Wavelength...]

7.1.5 [Auto Zero]

8. [Abs;%T Meter]

8.1 Buttons and Settings

10. Appendix

10.1 Spectra Manager Installation

Guide for installing the Spectra Manager software.

10.1.1 Before installation

10.1.2 Installing Spectra Manager from Floppy Disk

Step-by-step installation from floppy disk.

10.1.2.1 Starting up SETUP.EXE

10.1.2.2 Inputting operator name or company name

10.1.2.3 Setting the program directory

10.1.2.4 Setting the data directory

10.1.3 Installing Spectra Manager from CD-ROM

Installation guide using a CD-ROM.

10.2 Setting the Serial Port (RS-232 C)

Jasco V-550 Specifications

General

Model	V-550
Type	UV-Visible Spectrophotometer
Wavelength Range	190 - 900 nm
Wavelength Repeatability	±0.1 nm
Detector	Silicon photodiode
Baseline Flatness	±0.001 Abs
Photometric Accuracy	±0.002 Abs at 0.5 Abs
Photometric Repeatability	±0.001 Abs
Light Source	Deuterium Lamp and Tungsten Halogen Lamp
Monochromator	Czerny-Turner mount
Data Output	RS-232C
Sample Compartment	Standard 10 mm cell holder
Power Requirements	AC 100-240 V, 50/60 Hz
Wavelength Accuracy	±0.3 nm (at 656.1 nm D2 line), ±0.8 nm (Full range)
Stray Light	Less than 0.05% at 220 nm with NaI
Noise Level	0.0005 Abs